Spatial separation of Plk1 phosphorylation and activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00455583" target="_blank" >RIV/68378050:_____/15:00455583 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fonc.2015.00132" target="_blank" >http://dx.doi.org/10.3389/fonc.2015.00132</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fonc.2015.00132" target="_blank" >10.3389/fonc.2015.00132</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spatial separation of Plk1 phosphorylation and activity

Popis výsledku v původním jazyce

Polo-like kinase 1 (P1k1) is one of the major kinases controlling mitosis and cell division. Plk1 is first recruited to the centrosome in S phase, then appears on the kinetochores in late G2, and at the end of mitosis, it translocates to the central spindle. Activation of Plk1 requires phosphorylation of 1210 by Aurora A, an event that critically depends on the co-factor Bora. However, conflicting reports exist as to where Plk1 is first activated. Phosphorylation of 1210 is first observed at the centrosomes, but kinase activity seems to be restricted to the nucleus in the earlier phases of G2. Here, we demonstrate that Plk1 activity manifests itself first in the nucleus using a nuclear FRET-based biosensor for Plk1 activity. However, we find that Borais restricted to the cytoplasm and that Plk1 is phosphorylated on 1210 at the centrosomes. Our data demonstrate that while Plk1 activation occurs on centrosomes, downstream target phosphorylation by Plk1 first occurs in the nucleus. We di

Název v anglickém jazyce

Spatial separation of Plk1 phosphorylation and activity

Popis výsledku anglicky

Polo-like kinase 1 (P1k1) is one of the major kinases controlling mitosis and cell division. Plk1 is first recruited to the centrosome in S phase, then appears on the kinetochores in late G2, and at the end of mitosis, it translocates to the central spindle. Activation of Plk1 requires phosphorylation of 1210 by Aurora A, an event that critically depends on the co-factor Bora. However, conflicting reports exist as to where Plk1 is first activated. Phosphorylation of 1210 is first observed at the centrosomes, but kinase activity seems to be restricted to the nucleus in the earlier phases of G2. Here, we demonstrate that Plk1 activity manifests itself first in the nucleus using a nuclear FRET-based biosensor for Plk1 activity. However, we find that Borais restricted to the cytoplasm and that Plk1 is phosphorylated on 1210 at the centrosomes. Our data demonstrate that while Plk1 activation occurs on centrosomes, downstream target phosphorylation by Plk1 first occurs in the nucleus. We di

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-18392S" target="_blank" >GA13-18392S: Dynamika buněčné odpovědi na poškození DNA</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Oncology

ISSN

2234-943X

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

132

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000359144700001

EID výsledku v databázi Scopus

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