Nuclear Phosphoinositides Contribute to Organization of Transcription Sites and Regulation of Gene Expression

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nuclear Phosphoinositides Contribute to Organization of Transcription Sites and Regulation of Gene Expression

Popis výsledku v původním jazyce

The nucleus is a highly organized cell compartment, where controlled gene expression, DNA replication, and RNA processing occur. These processes are spatially ordered via the nucleoskeleton, which is involved in nuclear compartmentalization and critical for nuclear functioning. In spite of the growing interest and extensive research concerned to the nuclear organization, so far mostly protein complexes have been found as important for spatial nuclear ordering. We describe here novel structures containing phosphatidylinositol 4,5-bisphosphate (PIP2) which contribute as well. Based on scarce literature data relating to PIP2 presence in interchromatin granule clusters and in the nucleolus, we carried out ultrastructural mapping of pip2-containing structures using pre-embedding immunolabelling and 3D electron tomography, EELS and super-resolution fluorescence microscopy together with molecular biology experiments. We identified previously undescribed nucleoplasmic structures decorated by PIP2 molecules, and demonstrated that these structures, referred to as PIP2 islets, are evolutionary conserved. They are surrounded by nucleic acids and protein-containing compounds. At the islet periphery, PIP2 co-localizes with nascent RNA transcripts and the proteins engaged in Pol II transcription and organization of chromatin. PIP2 islets are sensitive to RNase treatment, and their enzymatic disruption affects the level of transcription. Additional data on islets attached molecular complexes will be presented. Based on our findings, we suggest that PIP2 islets play an important role in the organization of nuclear architecture providing multiple stable surfaces for the formation of the RNA polymerase I/II transcription complexes. Taken together, this data allowed us to suggest for the first time that PIP2 as a lipid representative plays an important role in the organization of chromatin architecture and in regulation of gene transcription. The result will be discussed in the fram...

Název v anglickém jazyce

Nuclear Phosphoinositides Contribute to Organization of Transcription Sites and Regulation of Gene Expression

Popis výsledku anglicky

The nucleus is a highly organized cell compartment, where controlled gene expression, DNA replication, and RNA processing occur. These processes are spatially ordered via the nucleoskeleton, which is involved in nuclear compartmentalization and critical for nuclear functioning. In spite of the growing interest and extensive research concerned to the nuclear organization, so far mostly protein complexes have been found as important for spatial nuclear ordering. We describe here novel structures containing phosphatidylinositol 4,5-bisphosphate (PIP2) which contribute as well. Based on scarce literature data relating to PIP2 presence in interchromatin granule clusters and in the nucleolus, we carried out ultrastructural mapping of pip2-containing structures using pre-embedding immunolabelling and 3D electron tomography, EELS and super-resolution fluorescence microscopy together with molecular biology experiments. We identified previously undescribed nucleoplasmic structures decorated by PIP2 molecules, and demonstrated that these structures, referred to as PIP2 islets, are evolutionary conserved. They are surrounded by nucleic acids and protein-containing compounds. At the islet periphery, PIP2 co-localizes with nascent RNA transcripts and the proteins engaged in Pol II transcription and organization of chromatin. PIP2 islets are sensitive to RNase treatment, and their enzymatic disruption affects the level of transcription. Additional data on islets attached molecular complexes will be presented. Based on our findings, we suggest that PIP2 islets play an important role in the organization of nuclear architecture providing multiple stable surfaces for the formation of the RNA polymerase I/II transcription complexes. Taken together, this data allowed us to suggest for the first time that PIP2 as a lipid representative plays an important role in the organization of chromatin architecture and in regulation of gene transcription. The result will be discussed in the fram...

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Cytogenetic and Genome Research

ISBN

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ISSN

1424-8581

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Karger

Místo vydání

Würzburg

Místo konání akce

Foz do Iguaçu

Datum konání akce

10. 7. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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