Whole-genome Expression Analysis in THP-1 Macrophage-like Cells Exposed to Diverse Nanomaterials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00490615" target="_blank" >RIV/68378050:_____/17:00490615 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/17:00490615

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Whole-genome Expression Analysis in THP-1 Macrophage-like Cells Exposed to Diverse Nanomaterials

Popis výsledku v původním jazyce

In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)] in THP-1 macrophage-like cells as a model cells of the first line of defense in the organism. Cells were incubated with non-cytotoxic concentrations (1-25 µg/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. NM-100 did not exert any significant effect, all other NMs triggered a pro-inflammatory response and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative stress as well as cell cycle progression and proteasome function.

Název v anglickém jazyce

Whole-genome Expression Analysis in THP-1 Macrophage-like Cells Exposed to Diverse Nanomaterials

Popis výsledku anglicky

In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)] in THP-1 macrophage-like cells as a model cells of the first line of defense in the organism. Cells were incubated with non-cytotoxic concentrations (1-25 µg/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. NM-100 did not exert any significant effect, all other NMs triggered a pro-inflammatory response and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative stress as well as cell cycle progression and proteasome function.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LO1508" target="_blank" >LO1508: Genomika a proteomika při studiu mechanismů biologických účinků vyráběných nanočástic</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Nanomaterials in Biomedical research

ISBN

978-80-906655-5-2

Počet stran výsledku

6

Strana od-do

9-14

Počet stran knihy

140

Název nakladatele

Proxima Tisk, s.r.o.

Místo vydání

Praha 3

Kód UT WoS kapitoly

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