Polymer cancerostatics targeted by recombinant antibody fragments to GD2-positive tumor cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00499714" target="_blank" >RIV/68378050:_____/19:00499714 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/19:00499714

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.biomac.8b01616" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.biomac.8b01616</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.biomac.8b01616" target="_blank" >10.1021/acs.biomac.8b01616</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polymer cancerostatics targeted by recombinant antibody fragments to GD2-positive tumor cells

Popis výsledku v původním jazyce

A water-soluble polymer cancerostatic actively targeted against cancer cells expressing a disialoganglioside antigen GD2 was designed, synthesized and characterized. A polymer conjugate of an antitumor drug doxorubicin with a N-(2-hydroxypropyl)methacrylamide-based copolymer was specifically targeted against GD2 antigen-positive tumor cells using a recombinant single chain fragment (scFv) of an anti-GD2 monoclonal antibody. The targeting protein ligand was attached to the polymer–drug conjugate either via a covalent bond between the amino groups of the protein using a traditional nonspecific aminolytic reaction with a reactive polymer precursor or via a noncovalent but highly specific interaction between bungarotoxin covalently linked to the polymer and the recombinant scFv modified with a C-terminal bungarotoxin-binding peptide. The GD2 antigen binding activity and GD2-specific cytotoxicity of the targeted noncovalent polymer–scFv complex proved to be superior to the covalent polymer–scFv conjugate.

Název v anglickém jazyce

Polymer cancerostatics targeted by recombinant antibody fragments to GD2-positive tumor cells

Popis výsledku anglicky

A water-soluble polymer cancerostatic actively targeted against cancer cells expressing a disialoganglioside antigen GD2 was designed, synthesized and characterized. A polymer conjugate of an antitumor drug doxorubicin with a N-(2-hydroxypropyl)methacrylamide-based copolymer was specifically targeted against GD2 antigen-positive tumor cells using a recombinant single chain fragment (scFv) of an anti-GD2 monoclonal antibody. The targeting protein ligand was attached to the polymer–drug conjugate either via a covalent bond between the amino groups of the protein using a traditional nonspecific aminolytic reaction with a reactive polymer precursor or via a noncovalent but highly specific interaction between bungarotoxin covalently linked to the polymer and the recombinant scFv modified with a C-terminal bungarotoxin-binding peptide. The GD2 antigen binding activity and GD2-specific cytotoxicity of the targeted noncovalent polymer–scFv complex proved to be superior to the covalent polymer–scFv conjugate.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomacromolecules

ISSN

1525-7797

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

412-421

Kód UT WoS článku

000456349600036

EID výsledku v databázi Scopus

2-s2.0-85059898451