Screening of fluorescence properties of chemical compounds using PerkinElmer EnSpire multi-mode plate reader

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00502881" target="_blank" >RIV/68378050:_____/19:00502881 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Screening of fluorescence properties of chemical compounds using PerkinElmer EnSpire multi-mode plate reader

Popis výsledku v původním jazyce

Determining of fluorescence properties of many compounds in parallel requires many spectral scans which requires automation. We were faced with a task of measuring fluorescence properties of a library of 123 fluorescent and colored compounds and finding compounds that change their fluorescence properties the most upon complexation with cyclodextrins. At our disposal for this was the EnSpire plate reader with fluorescence measurement and spectral scanning capabilities. Unfortunately, the software of the machine only supports measurement according to completely predefined protocols which do not allow the use of different parameters for each well of the plate or successive measurements with changing parameters. We have developed and made available a software enabling to change the measurement parameters and run the assays automatically from scripts.

Název v anglickém jazyce

Screening of fluorescence properties of chemical compounds using PerkinElmer EnSpire multi-mode plate reader

Popis výsledku anglicky

Determining of fluorescence properties of many compounds in parallel requires many spectral scans which requires automation. We were faced with a task of measuring fluorescence properties of a library of 123 fluorescent and colored compounds and finding compounds that change their fluorescence properties the most upon complexation with cyclodextrins. At our disposal for this was the EnSpire plate reader with fluorescence measurement and spectral scanning capabilities. Unfortunately, the software of the machine only supports measurement according to completely predefined protocols which do not allow the use of different parameters for each well of the plate or successive measurements with changing parameters. We have developed and made available a software enabling to change the measurement parameters and run the assays automatically from scripts.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

<a href="/cs/project/LO1220" target="_blank" >LO1220: CZ-OPENSCREEN: Národní infrastruktura chemické biologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Advances in Chemical Biology

ISBN

978-80-88011-03-3

Počet stran výsledku

4

Strana od-do

116-120

Počet stran knihy

210

Název nakladatele

OPTIO CZ

Místo vydání

Praha

Kód UT WoS kapitoly

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