SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00523243" target="_blank" >RIV/68378050:_____/19:00523243 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR

Popis výsledku v původním jazyce

The super-resolution (SR) methods are rapidly developing approaches in light microscopy field. The Stimulated emission depletion (STED) microscopy is SR method based on confocal scanning microscope. During the acquisition scanning, two lasers – excitation and depletion – are co-aligned into one single spot in the sample. For the maximal efficiency of the SR method, the proper co-alignment of used laser beams is crucial. The article explains the STED microscope operator recommended routine for the alignment. Simple experiments demonstrate significantly decreased number of incoming photons in the misaligned system. It naturally leads to the lower resolution during the acquisition and these important aspects are discussed within the article.

Název v anglickém jazyce

SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR

Popis výsledku anglicky

The super-resolution (SR) methods are rapidly developing approaches in light microscopy field. The Stimulated emission depletion (STED) microscopy is SR method based on confocal scanning microscope. During the acquisition scanning, two lasers – excitation and depletion – are co-aligned into one single spot in the sample. For the maximal efficiency of the SR method, the proper co-alignment of used laser beams is crucial. The article explains the STED microscope operator recommended routine for the alignment. Simple experiments demonstrate significantly decreased number of incoming photons in the misaligned system. It naturally leads to the lower resolution during the acquisition and these important aspects are discussed within the article.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/LO1419" target="_blank" >LO1419: Biomodely pro zdraví - Centrum modelových organismů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Advances in Disease Models

ISBN

978-80-88011-06-4

Počet stran výsledku

7

Strana od-do

234-240

Počet stran knihy

310

Název nakladatele

OPTIO CZ

Místo vydání

Praha

Kód UT WoS kapitoly

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