Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555844" target="_blank" >RIV/68378050:_____/21:00555844 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378050:_____/21:00555845
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy
Popis výsledku v původním jazyce
Current models of gene expression acknowledge protein clustering and formation of transcriptional condensates as a driving force of gene expression. These models are mostly based on single-molecule localization microscopy (SMLM) which provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the roles of nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, accumulating evidence suggests the involvement of nuclear lipids and particularly of phosphatidylinositol phosphates (PIPs) in gene expression. We used quantitative SMLM for the evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We showed phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) and phosphatidylinositol 4-monophosphate (PI(4)P) within nuclear speckles and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNA polymerase II (RNAPII) foci either in the nucleoplasm or nuclear speckles. We continue to uncover the PIP fingerprints in the subsequent stages of RNAPII transcription. Our efforts aim at elucidating the roles of nuclear PIPs in the compartmentalization of gene expression.
Název v anglickém jazyce
Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy
Popis výsledku anglicky
Current models of gene expression acknowledge protein clustering and formation of transcriptional condensates as a driving force of gene expression. These models are mostly based on single-molecule localization microscopy (SMLM) which provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the roles of nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, accumulating evidence suggests the involvement of nuclear lipids and particularly of phosphatidylinositol phosphates (PIPs) in gene expression. We used quantitative SMLM for the evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We showed phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) and phosphatidylinositol 4-monophosphate (PI(4)P) within nuclear speckles and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNA polymerase II (RNAPII) foci either in the nucleoplasm or nuclear speckles. We continue to uncover the PIP fingerprints in the subsequent stages of RNAPII transcription. Our efforts aim at elucidating the roles of nuclear PIPs in the compartmentalization of gene expression.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů