Behavioral Characterization of an Angelman Syndrome Mouse Model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00581626" target="_blank" >RIV/68378050:_____/23:00581626 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jove.com/t/65182/behavioral-characterization-of-an-angelman-syndrome-mouse-model" target="_blank" >https://www.jove.com/t/65182/behavioral-characterization-of-an-angelman-syndrome-mouse-model</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3791/65182" target="_blank" >10.3791/65182</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Behavioral Characterization of an Angelman Syndrome Mouse Model

Popis výsledku v původním jazyce

This manuscript describes a battery of behavioral tests available to characterize Angelman syndrome (AS)-like phenotypes in an established murine model of AS. We use the rotarod learning paradigm, detailed gait analysis, and nest building test to detect and characterize animal motor impairments. We test animal emotionality in the open field and elevated plus maze tests, as well as the affect in the tail suspension test. When AS mice are tested in the open field test, the results should be interpreted with care, since motor dysfunctions influence mouse behavior in the maze and alter activity scores. The reproducibility and effectiveness of the presented behavioral tests has already been validated in several independent Uba3a mouse lines with different knockout variants, establishing this set of tests as an excellent validation tool in AS research. Models with the relevant construct and face validity will warrant further investigations to elucidate the pathophysiology of the disease and grant the development of causal treatments.

Název v anglickém jazyce

Behavioral Characterization of an Angelman Syndrome Mouse Model

Popis výsledku anglicky

This manuscript describes a battery of behavioral tests available to characterize Angelman syndrome (AS)-like phenotypes in an established murine model of AS. We use the rotarod learning paradigm, detailed gait analysis, and nest building test to detect and characterize animal motor impairments. We test animal emotionality in the open field and elevated plus maze tests, as well as the affect in the tail suspension test. When AS mice are tested in the open field test, the results should be interpreted with care, since motor dysfunctions influence mouse behavior in the maze and alter activity scores. The reproducibility and effectiveness of the presented behavioral tests has already been validated in several independent Uba3a mouse lines with different knockout variants, establishing this set of tests as an excellent validation tool in AS research. Models with the relevant construct and face validity will warrant further investigations to elucidate the pathophysiology of the disease and grant the development of causal treatments.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Jove-Journal of Visualized Experiments

ISSN

1940-087X

e-ISSN

—

Svazek periodika

2023

Číslo periodika v rámci svazku

200

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

e65182

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85175006976