Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: <i>In vitro</i>-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00579877" target="_blank" >RIV/68378050:_____/24:00579877 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.spandidos-publications.com/10.3892/or.2023.8662" target="_blank" >https://www.spandidos-publications.com/10.3892/or.2023.8662</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2023.8662" target="_blank" >10.3892/or.2023.8662</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: <i>In vitro</i>-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling

Popis výsledku v původním jazyce

Breast cancer is the most frequently diagnosed cancer in women worldwide. Although dramatically increased survival rates of early diagnosed cases have been observed, late diagnosed patients and metastatic cancer may still be considered fatal. The present study's main focus was on cancer-associated fibroblasts (CAFs) which is an active component of the tumor microenvironment (TME) regulating the breast cancer ecosystem. Transcriptomic profiling and analysis of CAFs isolated from breast cancer skin metastasis, cutaneous basal cell carcinoma, and squamous cell carcinoma unravelled major gene candidates such as IL6, VEGFA and MFGE8 that induced co-expression of keratins-8/-14 in the EM-G3 cell line derived from infiltrating ductal breast carcinoma. Western blot analysis of selected keratins (keratin-8,14,18,19) and epithelial-mesenchymal transition-associated markers (SLUG, SNAIL, ZEB1, E-/N-cadherin, vimentin) revealed specific responses pointing to certain heterogeneity of the studied CAF populations. Experimental in vitro treatment using neutralizing antibodies against IL-6, VEGF-A and MFGE8 attenuated the modulatory effect of CAFs on EM-G3 cells. The present study provided novel data in characterizing and understanding the interactions between CAFs and EM-G3 cells in vitro. CAFs of different origins support the pro-inflammatory microenvironment and influence the biology of breast cancer cells. This observation potentially holds significant interest for the development of novel, clinically relevant approaches targeting the TME in breast cancer. Furthermore, its implications extend beyond breast cancer and have the potential to impact a wide range of other cancer types.

Název v anglickém jazyce

Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: <i>In vitro</i>-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling

Popis výsledku anglicky

Breast cancer is the most frequently diagnosed cancer in women worldwide. Although dramatically increased survival rates of early diagnosed cases have been observed, late diagnosed patients and metastatic cancer may still be considered fatal. The present study's main focus was on cancer-associated fibroblasts (CAFs) which is an active component of the tumor microenvironment (TME) regulating the breast cancer ecosystem. Transcriptomic profiling and analysis of CAFs isolated from breast cancer skin metastasis, cutaneous basal cell carcinoma, and squamous cell carcinoma unravelled major gene candidates such as IL6, VEGFA and MFGE8 that induced co-expression of keratins-8/-14 in the EM-G3 cell line derived from infiltrating ductal breast carcinoma. Western blot analysis of selected keratins (keratin-8,14,18,19) and epithelial-mesenchymal transition-associated markers (SLUG, SNAIL, ZEB1, E-/N-cadherin, vimentin) revealed specific responses pointing to certain heterogeneity of the studied CAF populations. Experimental in vitro treatment using neutralizing antibodies against IL-6, VEGF-A and MFGE8 attenuated the modulatory effect of CAFs on EM-G3 cells. The present study provided novel data in characterizing and understanding the interactions between CAFs and EM-G3 cells in vitro. CAFs of different origins support the pro-inflammatory microenvironment and influence the biology of breast cancer cells. This observation potentially holds significant interest for the development of novel, clinically relevant approaches targeting the TME in breast cancer. Furthermore, its implications extend beyond breast cancer and have the potential to impact a wide range of other cancer types.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Oncology Reports

ISSN

1021-335X

e-ISSN

1791-2431

Svazek periodika

51

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

12

Strana od-do

3

Kód UT WoS článku

001111760900001

EID výsledku v databázi Scopus

2-s2.0-85177454254