Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00586779" target="_blank" >RIV/68378050:_____/24:00586779 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10481931 RIV/60461373:22310/24:43930346 RIV/60461373:22330/24:43930346 RIV/00064165:_____/24:10481931

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-024-62615-w" target="_blank" >https://www.nature.com/articles/s41598-024-62615-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-024-62615-w" target="_blank" >10.1038/s41598-024-62615-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro

Popis výsledku v původním jazyce

Statins, the drugs used for the treatment of hypercholesterolemia, have come into the spotlight not only as chemoadjuvants, but also as potential stem cell modulators in the context of regenerative therapy. In our study, we compared the in vitro effects of all clinically used statins on the viability of human pancreatic cancer (MiaPaCa-2) cells, non-cancerous human embryonic kidney (HEK 293) cells and adipose-derived mesenchymal stem cells (ADMSC). Additionally, the effect of statins on viability of MiaPaCa-2 and ADMSC cells spheroids was tested. Furthermore, we performed a microarray analysis on ADMSCs treated with individual statins (12 mu M) and compared the importance of the effects of statins on gene expression between stem cells and pancreatic cancer cells. Concentrations of statins that significantly affected cancer cells viability (< 40 mu M) did not affect stem cells viability after 24 h. Moreover, statins that didn't affect viability of cancer cells grown in a monolayer, induce the disintegration of cancer cell spheroids. The effect of statins on gene expression was significantly less pronounced in stem cells compared to pancreatic cancer cells. In conclusion, the low efficacy of statins on non-tumor and stem cells at concentrations sufficient for cancer cells growth inhibition, support their applicability in chemoadjuvant tumor therapy.

Název v anglickém jazyce

Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro

Popis výsledku anglicky

Statins, the drugs used for the treatment of hypercholesterolemia, have come into the spotlight not only as chemoadjuvants, but also as potential stem cell modulators in the context of regenerative therapy. In our study, we compared the in vitro effects of all clinically used statins on the viability of human pancreatic cancer (MiaPaCa-2) cells, non-cancerous human embryonic kidney (HEK 293) cells and adipose-derived mesenchymal stem cells (ADMSC). Additionally, the effect of statins on viability of MiaPaCa-2 and ADMSC cells spheroids was tested. Furthermore, we performed a microarray analysis on ADMSCs treated with individual statins (12 mu M) and compared the importance of the effects of statins on gene expression between stem cells and pancreatic cancer cells. Concentrations of statins that significantly affected cancer cells viability (< 40 mu M) did not affect stem cells viability after 24 h. Moreover, statins that didn't affect viability of cancer cells grown in a monolayer, induce the disintegration of cancer cell spheroids. The effect of statins on gene expression was significantly less pronounced in stem cells compared to pancreatic cancer cells. In conclusion, the low efficacy of statins on non-tumor and stem cells at concentrations sufficient for cancer cells growth inhibition, support their applicability in chemoadjuvant tumor therapy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/EF16_019%2F0000785" target="_blank" >EF16_019/0000785: Centrum nádorové ekologie - výzkum nádorového mikroprostředí v organizmu podporujícího růst a šíření nádoru</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

11830

Kód UT WoS článku

001230489600005

EID výsledku v databázi Scopus

2-s2.0-85194126241