Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00618822" target="_blank" >RIV/68378050:_____/25:00618822 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.ydbio.2025.03.008" target="_blank" >https://doi.org/10.1016/j.ydbio.2025.03.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ydbio.2025.03.008" target="_blank" >10.1016/j.ydbio.2025.03.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion

Popis výsledku v původním jazyce

Optic cup morphogenesis is a complex process involving cellular behaviors such as epithelial folding, cell shape changes, proliferation, and tissue fusion. Disruptions to these processes can lead to an ocular coloboma, a congenital defect where the optic fissure fails to close. This study investigates the role of Shroom3, a protein implicated in epithelial morphogenesis, in mouse embryos during optic cup development. It was observed that Shroom3 is apically localized in the neural retina and retinal pigmented epithelium, and its deficiency leads to a both a conventional coloboma phenotype characterized by a gap in pigmented tissue as well as a unique type of coloboma where an ectopic ventral fold of neural tissue is present. Increased apical areas of both neural retina and retinal pigmented epithelial cells are present in the absence of Shroom3 leading to a greater apical surface area and disruption of optic fissure alignment. Neural retina specific gene ablation revealed that Shroom3 function in the RPE is likely sufficient to facilitate tissue alignment and permit fusion. However, the fusion process is ultimately disturbed due to a failure of the neural tissue to reestablish apical-basal polarity. Furthermore, it is demonstrated that Shroom3 deficiency also affects other epithelial fusion events in the embryo that rely on polarity reestablishment, such as lens vesicle separation, eyelid formation, and secondary palate closure. These findings highlight the importance of Shroom3 during optic cup morphogenesis, aid our understanding of optic fissure closure and coloboma formation, and implicates a role for Shroom3 in regulating apical-basal polarity.

Název v anglickém jazyce

Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion

Popis výsledku anglicky

Optic cup morphogenesis is a complex process involving cellular behaviors such as epithelial folding, cell shape changes, proliferation, and tissue fusion. Disruptions to these processes can lead to an ocular coloboma, a congenital defect where the optic fissure fails to close. This study investigates the role of Shroom3, a protein implicated in epithelial morphogenesis, in mouse embryos during optic cup development. It was observed that Shroom3 is apically localized in the neural retina and retinal pigmented epithelium, and its deficiency leads to a both a conventional coloboma phenotype characterized by a gap in pigmented tissue as well as a unique type of coloboma where an ectopic ventral fold of neural tissue is present. Increased apical areas of both neural retina and retinal pigmented epithelial cells are present in the absence of Shroom3 leading to a greater apical surface area and disruption of optic fissure alignment. Neural retina specific gene ablation revealed that Shroom3 function in the RPE is likely sufficient to facilitate tissue alignment and permit fusion. However, the fusion process is ultimately disturbed due to a failure of the neural tissue to reestablish apical-basal polarity. Furthermore, it is demonstrated that Shroom3 deficiency also affects other epithelial fusion events in the embryo that rely on polarity reestablishment, such as lens vesicle separation, eyelid formation, and secondary palate closure. These findings highlight the importance of Shroom3 during optic cup morphogenesis, aid our understanding of optic fissure closure and coloboma formation, and implicates a role for Shroom3 in regulating apical-basal polarity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/GA21-27364S" target="_blank" >GA21-27364S: Role transkripčních faktorů ve vývoji očí: vhled z genetiky, transkriptomiky a regulačních krajin.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Biology

ISSN

0012-1606

e-ISSN

1095-564X

Svazek periodika

522

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

91-105

Kód UT WoS článku

001457105900001

EID výsledku v databázi Scopus

2-s2.0-105000736357