Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00645548" target="_blank" >RIV/68378050:_____/25:00645548 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-4522-2_11" target="_blank" >https://doi.org/10.1007/978-1-0716-4522-2_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-4522-2_11" target="_blank" >10.1007/978-1-0716-4522-2_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos

Popis výsledku v původním jazyce

MicroRNAs (miRNAs), genome-encoded small RNAs associated with Argonaute proteins, are important negative regulators of gene expression in mammalian cells. miRNAs usually partially base pair with mRNAs, suppress their translation, and destabilize them. Sufficient miRNA abundance is an important factor for efficient target repression. Experimental evidence suggests that oocyte growth causes a dilution effect, which reduces concentrations of maternal miRNAs and renders them functionally inefficient. Consequently, efficient target repression is retained only by those maternal miRNAs which achieve a favorable miRNA:mRNA stoichiometry. Here, we provide protocols for PCR-based quantification of miRNAs and luciferase reporter-based analysis of their activity in mammalian oocytes and early embryos.

Název v anglickém jazyce

Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos

Popis výsledku anglicky

MicroRNAs (miRNAs), genome-encoded small RNAs associated with Argonaute proteins, are important negative regulators of gene expression in mammalian cells. miRNAs usually partially base pair with mRNAs, suppress their translation, and destabilize them. Sufficient miRNA abundance is an important factor for efficient target repression. Experimental evidence suggests that oocyte growth causes a dilution effect, which reduces concentrations of maternal miRNAs and renders them functionally inefficient. Consequently, efficient target repression is retained only by those maternal miRNAs which achieve a favorable miRNA:mRNA stoichiometry. Here, we provide protocols for PCR-based quantification of miRNAs and luciferase reporter-based analysis of their activity in mammalian oocytes and early embryos.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA pro terapii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Zygotic Genome Activation

ISBN

978-1-0716-4522-2

Počet stran výsledku

13

Strana od-do

181-194

Počet stran knihy

264

Název nakladatele

Humana

Místo vydání

New York

Kód UT WoS kapitoly

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