The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F16%3A00467466" target="_blank" >RIV/68378271:_____/16:00467466 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/16:00467466 RIV/61389013:_____/16:00467466 RIV/00216208:11110/16:10329575 RIV/00216208:11130/16:10329575 RIV/00023001:_____/16:00060103

Výsledek na webu

<a href="http://dx.doi.org/10.2147/IJN.S116171" target="_blank" >http://dx.doi.org/10.2147/IJN.S116171</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S116171" target="_blank" >10.2147/IJN.S116171</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Popis výsledku v původním jazyce

Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated gamma-Fe2O3) and studied their effect on proliferation and neuronal differentiation. nnMaterials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. nnResults: Cell proliferation was not affected by PLL-coated gamma-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated gamma-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. nnConclusion: Our results show that cells labeled with PLL-coated gamma-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.

Název v anglickém jazyce

The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Popis výsledku anglicky

Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated gamma-Fe2O3) and studied their effect on proliferation and neuronal differentiation. nnMaterials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. nnResults: Cell proliferation was not affected by PLL-coated gamma-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated gamma-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. nnConclusion: Our results show that cells labeled with PLL-coated gamma-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

2016

Stát vydavatele periodika

NZ - Nový Zéland

Počet stran výsledku

15

Strana od-do

6267-6281

Kód UT WoS článku

000388405800001

EID výsledku v databázi Scopus

2-s2.0-85000868493