Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00504656" target="_blank" >RIV/68378271:_____/19:00504656 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/19:00504656 RIV/68407700:21110/19:00327328 RIV/68407700:21230/19:00327328 RIV/68407700:21460/19:00327328

Výsledek na webu

<a href="https://doi.org/10.1016/j.colsurfb.2019.01.048" target="_blank" >https://doi.org/10.1016/j.colsurfb.2019.01.048</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2019.01.048" target="_blank" >10.1016/j.colsurfb.2019.01.048</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells

Popis výsledku v původním jazyce

Here we present a diamond-based impedance sensor with built-in gold IDT as a promising platform for simultaneous electrical and optical monitoring of adipose tissue-derived stem cells (ASCs). The impedance spectra were collected in a wide frequency from 100 Hz to 50 kHz for 90 h of cell cultivation. Absolute impedance spectra were analyzed in terms of cell properties monitored by a high-resolution digital camera. The sensors detected three phases of cell growth: the phase of cell attachment and spreading, the phase of cell proliferation, and the stationary phase without significant changes in cell number. These results were confirmed by simultaneous live cell imaging. The design of the sensing electrode is discussed, pointing out its enhanced sensitivity for a certain case. The diamond-based sensor appeared to be more sensitive for detecting the cell-substrate interaction in the first phase of cell growth, while the control system was more sensitive in the second phase of cell growth.

Název v anglickém jazyce

Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells

Popis výsledku anglicky

Here we present a diamond-based impedance sensor with built-in gold IDT as a promising platform for simultaneous electrical and optical monitoring of adipose tissue-derived stem cells (ASCs). The impedance spectra were collected in a wide frequency from 100 Hz to 50 kHz for 90 h of cell cultivation. Absolute impedance spectra were analyzed in terms of cell properties monitored by a high-resolution digital camera. The sensors detected three phases of cell growth: the phase of cell attachment and spreading, the phase of cell proliferation, and the stationary phase without significant changes in cell number. These results were confirmed by simultaneous live cell imaging. The design of the sensing electrode is discussed, pointing out its enhanced sensitivity for a certain case. The diamond-based sensor appeared to be more sensitive for detecting the cell-substrate interaction in the first phase of cell growth, while the control system was more sensitive in the second phase of cell growth.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

177

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

130-136

Kód UT WoS článku

000468720700015

EID výsledku v databázi Scopus

2-s2.0-85060851825