Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F20%3A00543374" target="_blank" >RIV/68378271:_____/20:00543374 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0320589" target="_blank" >http://hdl.handle.net/11104/0320589</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41597-020-00729-2" target="_blank" >10.1038/s41597-020-00729-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs

Popis výsledku v původním jazyce

Structural studies of challenging targets such as G protein-coupled receptors (GPCRs) have accelerated during the last several years due to the development of new approaches, including small-wedge and serial crystallography. Here, we describe the deposition of seven datasets consisting of X-ray diffraction images acquired from lipidic cubic phase (LCP) grown microcrystals of two human GPCRs, Cysteinyl leukotriene receptors 1 and 2 (CysLT(1)R and CysLT(2)R), in complex with various antagonists. Five datasets were collected using small-wedge synchrotron crystallography (SWSX) at the European Synchrotron Radiation Facility with multiple crystals under cryo-conditions. Two datasets were collected using X-ray free electron laser (XFEL) serial femtosecond crystallography (SFX) at the Linac Coherent Light Source, with microcrystals delivered at room temperature into the beam within LCP matrix by a viscous media microextrusion injector.n

Název v anglickém jazyce

Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs

Popis výsledku anglicky

Structural studies of challenging targets such as G protein-coupled receptors (GPCRs) have accelerated during the last several years due to the development of new approaches, including small-wedge and serial crystallography. Here, we describe the deposition of seven datasets consisting of X-ray diffraction images acquired from lipidic cubic phase (LCP) grown microcrystals of two human GPCRs, Cysteinyl leukotriene receptors 1 and 2 (CysLT(1)R and CysLT(2)R), in complex with various antagonists. Five datasets were collected using small-wedge synchrotron crystallography (SWSX) at the European Synchrotron Radiation Facility with multiple crystals under cryo-conditions. Two datasets were collected using X-ray free electron laser (XFEL) serial femtosecond crystallography (SFX) at the Linac Coherent Light Source, with microcrystals delivered at room temperature into the beam within LCP matrix by a viscous media microextrusion injector.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Data

ISSN

2052-4463

e-ISSN

2052-4463

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

388

Kód UT WoS článku

000593909300001

EID výsledku v databázi Scopus

2-s2.0-85095948049