DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00566160" target="_blank" >RIV/68378271:_____/21:00566160 - isvavai.cz</a>

Výsledek na webu

<a href="http://doi.org/10.1080/09553002.2021.1948140" target="_blank" >http://doi.org/10.1080/09553002.2021.1948140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/09553002.2021.1948140" target="_blank" >10.1080/09553002.2021.1948140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time

Popis výsledku v původním jazyce

Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional gamma-rays and high LET carbon ions were also performed. Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit.

Název v anglickém jazyce

DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time

Popis výsledku anglicky

Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional gamma-rays and high LET carbon ions were also performed. Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10303 - Particles and field physics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Radiation Biology

ISSN

0955-3002

e-ISSN

1362-3095

Svazek periodika

97

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1229-1240

Kód UT WoS článku

000674807500001

EID výsledku v databázi Scopus

2-s2.0-85110868328