Scalable production of tissue-like vascularized liver organoids from human PSCs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F23%3A00576064" target="_blank" >RIV/68378271:_____/23:00576064 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/23:10469520

Výsledek na webu

<a href="https://hdl.handle.net/11104/0346848" target="_blank" >https://hdl.handle.net/11104/0346848</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s12276-023-01074-1" target="_blank" >10.1038/s12276-023-01074-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Scalable production of tissue-like vascularized liver organoids from human PSCs

Popis výsledku v původním jazyce

The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality.

Název v anglickém jazyce

Scalable production of tissue-like vascularized liver organoids from human PSCs

Popis výsledku anglicky

The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental and Molecular Medicine

ISSN

1226-3613

e-ISSN

2092-6413

Svazek periodika

55

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

20

Strana od-do

2005-2024

Kód UT WoS článku

001065142700006

EID výsledku v databázi Scopus

2-s2.0-85169166859