Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F24%3A00599586" target="_blank" >RIV/68378271:_____/24:00599586 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/24:10494002

Výsledek na webu

<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12999/3017613/Effects-of-storage-on-stability-and-performance-of-carboxybetaine-based/10.1117/12.3017613.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12999/3017613/Effects-of-storage-on-stability-and-performance-of-carboxybetaine-based/10.1117/12.3017613.short</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.3017613" target="_blank" >10.1117/12.3017613</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Popis výsledku v původním jazyce

Zwitterionic poly(carboxybetaine) brushes have emerged as promising surface functional coatings for antifouling label-free biosensing in complex biological fluids. This study investigates the effect of storage conditions on highly-dense poly(carboxybetaine methacrylamide) (pCBMAA), poly(sulfobetaine methacrylamide) (pSBMAA), a random copolymer of CBMAA with poly(hydroxypropyl methacrylamide) p(CBMAA-HPMAA), and terpolymer p(CBMAA-SBMAA-HPMAA) brushes. Synthesized via surface-initiated atom transfer radical polymerization (SI ATRP) on gold-coated substrates, these brushes were stored in dry, water, or phosphate-buffered saline (PBS) environments at temperatures of 22 °C, 6 °C, or frozen at –20 °C for 44 days. Structural stability of the brushes was assessed using infrared reflection absorption spectroscopy (IRRAS) and spectroscopic ellipsometry (SE), while fouling resistance and IgG antibody binding capacity were evaluated using surface plasmon resonance (SPR). Although the brushes exhibited minimal detachment from the surface, a slight decrease in swelling ratio was observed across all storage conditions. Furthermore, the storage led to a minor reduction in IgG binding capacity, with freezing at –20 °C showing a more pronounced effect in most cases. Notably, storing all tested carboxybetaine-based brushes in water or in PBS at 6 °C adversely affected fouling, a phenomenon alleviated when stored frozen at –20 °C or in a dry state.

Název v anglickém jazyce

Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Popis výsledku anglicky

Zwitterionic poly(carboxybetaine) brushes have emerged as promising surface functional coatings for antifouling label-free biosensing in complex biological fluids. This study investigates the effect of storage conditions on highly-dense poly(carboxybetaine methacrylamide) (pCBMAA), poly(sulfobetaine methacrylamide) (pSBMAA), a random copolymer of CBMAA with poly(hydroxypropyl methacrylamide) p(CBMAA-HPMAA), and terpolymer p(CBMAA-SBMAA-HPMAA) brushes. Synthesized via surface-initiated atom transfer radical polymerization (SI ATRP) on gold-coated substrates, these brushes were stored in dry, water, or phosphate-buffered saline (PBS) environments at temperatures of 22 °C, 6 °C, or frozen at –20 °C for 44 days. Structural stability of the brushes was assessed using infrared reflection absorption spectroscopy (IRRAS) and spectroscopic ellipsometry (SE), while fouling resistance and IgG antibody binding capacity were evaluated using surface plasmon resonance (SPR). Although the brushes exhibited minimal detachment from the surface, a slight decrease in swelling ratio was observed across all storage conditions. Furthermore, the storage led to a minor reduction in IgG binding capacity, with freezing at –20 °C showing a more pronounced effect in most cases. Notably, storing all tested carboxybetaine-based brushes in water or in PBS at 6 °C adversely affected fouling, a phenomenon alleviated when stored frozen at –20 °C or in a dry state.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of SPIE

ISBN

978-151067316-8

ISSN

0277-786X

e-ISSN

—

Počet stran výsledku

6

Strana od-do

129990O

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

Strasbourg

Datum konání akce

7. 4. 2024

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

001281471700019