Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F24%3A00601124" target="_blank" >RIV/68378271:_____/24:00601124 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15640/24:73628833 RIV/68407700:21340/24:00378049

Výsledek na webu

<a href="https://hdl.handle.net/11104/0358369" target="_blank" >https://hdl.handle.net/11104/0358369</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-024-53555-0" target="_blank" >10.1038/s41467-024-53555-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM

Popis výsledku v původním jazyce

Glycans are a major class of biopolymers, essential to all living organisms. To better understand their biological functions and applications in biomedicine or materials science, detailed knowledge of their structure is essential. However, due to their extraordinary complexity and conformational flexibility, state-of-the-art glycan analysis methods often fail to provide structural information with atomic precision. We combine electrospray deposition in ultra-high vacuum with non-contact atomic force microscopy and theoretical calculations to unravel the structure of β-cyclodextrin, a cyclic glucose oligomer, with atomic-scale detail. Our results reveal different adsorption geometries and conformations of β-cyclodextrin molecules. The position of individual hydroxyl groups and the location of the stabilizing intramolecular H-bonds are deduced from atomically resolved images, demonstrating the potential of the method for glycan analysis.

Název v anglickém jazyce

Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM

Popis výsledku anglicky

Glycans are a major class of biopolymers, essential to all living organisms. To better understand their biological functions and applications in biomedicine or materials science, detailed knowledge of their structure is essential. However, due to their extraordinary complexity and conformational flexibility, state-of-the-art glycan analysis methods often fail to provide structural information with atomic precision. We combine electrospray deposition in ultra-high vacuum with non-contact atomic force microscopy and theoretical calculations to unravel the structure of β-cyclodextrin, a cyclic glucose oligomer, with atomic-scale detail. Our results reveal different adsorption geometries and conformations of β-cyclodextrin molecules. The position of individual hydroxyl groups and the location of the stabilizing intramolecular H-bonds are deduced from atomically resolved images, demonstrating the potential of the method for glycan analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Communications

ISSN

2041-1723

e-ISSN

2041-1723

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

9482

Kód UT WoS článku

001346923200021

EID výsledku v databázi Scopus

2-s2.0-85208290696