Facile fabrication of large-area biofunctionalized metallic nanostructures for high performance affinity plasmonic biosensors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00635551" target="_blank" >RIV/68378271:_____/25:00635551 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0366613" target="_blank" >https://hdl.handle.net/11104/0366613</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/admt.202500356" target="_blank" >10.1002/admt.202500356</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Facile fabrication of large-area biofunctionalized metallic nanostructures for high performance affinity plasmonic biosensors

Popis výsledku v původním jazyce

An approach is presented to the facile preparation of biointerface-caped periodic arrays of gold nanoparticles (Au NP) with controlled plasmonic and advanced sensing properties. It relies on feedback-controlled UV-laser interference lithography combined with a novel ion milling method. These advancements improved preparation reproducibility yielding localized surface plasmon resonance (LSPR) wavelength tuned to near infrared spectrum with standard deviation of 10 nm. Moreover, a novel molecular toolkit of thiols and silatrane linkers with carboxybetaine/sulfobetaine headgroups is employed for orthogonal chemical modification of Au NPs and passivation of the optically non-active sensor chip regions, to prevent non-specific sorption of molecules from liquid samples. As part of this toolbox, the use of mercaptopropyl silatrane is reported as an adhesion-promoting coating for attaching Au NPs to an oxide substrate, replacing the traditionally used strongly absorbing chromium. The developed biofunctional optical nanostructures show LSPR with improved quality factor and enable 2.5-fold increase in accuracy for tracking of biomolecular binding on their surface via LSPR wavelength variations monitoring. In addition, a strategy is demonstrated to mitigate unspecific biomolecule sorption not only at the plasmonic hotspot on the metal surface, but also on the often-overlooked oxide substrate, highlighting the importance of the described orthogonal modification.

Název v anglickém jazyce

Facile fabrication of large-area biofunctionalized metallic nanostructures for high performance affinity plasmonic biosensors

Popis výsledku anglicky

An approach is presented to the facile preparation of biointerface-caped periodic arrays of gold nanoparticles (Au NP) with controlled plasmonic and advanced sensing properties. It relies on feedback-controlled UV-laser interference lithography combined with a novel ion milling method. These advancements improved preparation reproducibility yielding localized surface plasmon resonance (LSPR) wavelength tuned to near infrared spectrum with standard deviation of 10 nm. Moreover, a novel molecular toolkit of thiols and silatrane linkers with carboxybetaine/sulfobetaine headgroups is employed for orthogonal chemical modification of Au NPs and passivation of the optically non-active sensor chip regions, to prevent non-specific sorption of molecules from liquid samples. As part of this toolbox, the use of mercaptopropyl silatrane is reported as an adhesion-promoting coating for attaching Au NPs to an oxide substrate, replacing the traditionally used strongly absorbing chromium. The developed biofunctional optical nanostructures show LSPR with improved quality factor and enable 2.5-fold increase in accuracy for tracking of biomolecular binding on their surface via LSPR wavelength variations monitoring. In addition, a strategy is demonstrated to mitigate unspecific biomolecule sorption not only at the plasmonic hotspot on the metal surface, but also on the often-overlooked oxide substrate, highlighting the importance of the described orthogonal modification.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Materials Technologies

ISSN

2365-709X

e-ISSN

2365-709X

Svazek periodika

10

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

e00356

Kód UT WoS článku

001490966000001

EID výsledku v databázi Scopus

2-s2.0-105005774815