Fabrication, characterization and in vitro biocompatibility of electrospun hydroxyethyl cellulose/poly (vinyl) alcohol nanofibrous composite biomaterial for bone tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21110%2F16%3A00237601" target="_blank" >RIV/68407700:21110/16:00237601 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0009250916000269" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0009250916000269</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ces.2015.12.030" target="_blank" >10.1016/j.ces.2015.12.030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fabrication, characterization and in vitro biocompatibility of electrospun hydroxyethyl cellulose/poly (vinyl) alcohol nanofibrous composite biomaterial for bone tissue engineering

Popis výsledku v původním jazyce

Development of novel scaffold materials that mimic the extracellular matrix, architecturally and functionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose (HEC) based nanostructured scaffolds with uniform fiber morphology through electrospinning. Poly (vinyl alcohol) (PVA) was used as an ionic solvent for supporting the electrospinning of HEC. Scanning electron microscopy and ImageJ analysis revealed the formation of non-woven nanofibers with well-defined porous architecture. The interactions between HEC and PVA in the electrospun nanofibers were studied by differential scanning calorimetry, X-ray diffraction, dynamic mechanical analysis thermo-gravimetric analysis; Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and tensile test. The mechanical properties of scaffolds were significantly altered with different ratios of HEC/PVA. Further, the biocompatibility of HEC/PVA scaffolds was evaluated using human osteosarcoma cells. The SEM images revealed favorable cells attachment and spreading on the nanofibrous scaffolds and MTS assay showed increased cell proliferation after different time periods. Thus, these results indicate that HEC based nanofibrous scaffolds will be a promising candidate for bone tissue engineering.

Název v anglickém jazyce

Fabrication, characterization and in vitro biocompatibility of electrospun hydroxyethyl cellulose/poly (vinyl) alcohol nanofibrous composite biomaterial for bone tissue engineering

Popis výsledku anglicky

Development of novel scaffold materials that mimic the extracellular matrix, architecturally and functionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose (HEC) based nanostructured scaffolds with uniform fiber morphology through electrospinning. Poly (vinyl alcohol) (PVA) was used as an ionic solvent for supporting the electrospinning of HEC. Scanning electron microscopy and ImageJ analysis revealed the formation of non-woven nanofibers with well-defined porous architecture. The interactions between HEC and PVA in the electrospun nanofibers were studied by differential scanning calorimetry, X-ray diffraction, dynamic mechanical analysis thermo-gravimetric analysis; Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and tensile test. The mechanical properties of scaffolds were significantly altered with different ratios of HEC/PVA. Further, the biocompatibility of HEC/PVA scaffolds was evaluated using human osteosarcoma cells. The SEM images revealed favorable cells attachment and spreading on the nanofibrous scaffolds and MTS assay showed increased cell proliferation after different time periods. Thus, these results indicate that HEC based nanofibrous scaffolds will be a promising candidate for bone tissue engineering.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JN - Stavebnictví

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0034" target="_blank" >EE2.3.30.0034: Podpora zkvalitnění týmů výzkumu a vývoje a rozvoj intersektorální mobility na ČVUT v Praze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Engineering Science

ISSN

0009-2509

e-ISSN

—

Svazek periodika

144

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

17-29

Kód UT WoS článku

000371472700003

EID výsledku v databázi Scopus

2-s2.0-84955611749