The effect of temperature on bacterial self-healing processes in building materials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21110%2F20%3A00337886" target="_blank" >RIV/68407700:21110/20:00337886 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1088/1757-899X/726/1/012012" target="_blank" >https://doi.org/10.1088/1757-899X/726/1/012012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1757-899X/726/1/012012" target="_blank" >10.1088/1757-899X/726/1/012012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of temperature on bacterial self-healing processes in building materials

Popis výsledku v původním jazyce

This paper is focused on the bacterial induced calcitation for the crack healing. The bacteria applied for this purpose are from group which is adapted for growth in the high pH environment as a concrete in hydration phase and their metabolic activity leads to create of calcite. In this study, three different bacteria strains (Sporosarcina pasteurii, Bacillus cohnii, Bacillus pseudofirmus) were applied and the influence of various temperatures on their microbial properties was investigated. Our previous experiment indicated that one of the applied bacterial strain in spores form (Bacillus pseudofirmus) are able to survive the temperatures in the range from -20 °C to 140 °C. The xperiment described in this paper extends the previous study and determines the effect of different temperatures on the change in growth activity. In this experiment, bacterial activity was determined based on the change of absorbance in 640 nm by spectrophotometric measurements. The experiment was performed at optimal temperature (30 °C) and lower temperature (10 °C) and it used suitable broth for calcitation. The results showed that the beginning of metabolic activity was shifted by 40 to 50 hours. Only Bacillus cohnii showed different results because its metabolic activity was nearly zero at 10 °C.

Název v anglickém jazyce

The effect of temperature on bacterial self-healing processes in building materials

Popis výsledku anglicky

This paper is focused on the bacterial induced calcitation for the crack healing. The bacteria applied for this purpose are from group which is adapted for growth in the high pH environment as a concrete in hydration phase and their metabolic activity leads to create of calcite. In this study, three different bacteria strains (Sporosarcina pasteurii, Bacillus cohnii, Bacillus pseudofirmus) were applied and the influence of various temperatures on their microbial properties was investigated. Our previous experiment indicated that one of the applied bacterial strain in spores form (Bacillus pseudofirmus) are able to survive the temperatures in the range from -20 °C to 140 °C. The xperiment described in this paper extends the previous study and determines the effect of different temperatures on the change in growth activity. In this experiment, bacterial activity was determined based on the change of absorbance in 640 nm by spectrophotometric measurements. The experiment was performed at optimal temperature (30 °C) and lower temperature (10 °C) and it used suitable broth for calcitation. The results showed that the beginning of metabolic activity was shifted by 40 to 50 hours. Only Bacillus cohnii showed different results because its metabolic activity was nearly zero at 10 °C.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GA18-15697S" target="_blank" >GA18-15697S: Samohojení cementových kompozitů v důsledku bakteriální kalcifikace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

IOP Conference series: Materials Science and Engineering

ISBN

—

ISSN

1757-8981

e-ISSN

1757-899X

Počet stran výsledku

5

Strana od-do

—

Název nakladatele

IOPscience

Místo vydání

Madeira

Místo konání akce

Nová Lesná

Datum konání akce

2. 9. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000539289000012