Viability of yeasts after exposure to flax-fiber based composite materials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21110%2F23%3A00369938" target="_blank" >RIV/68407700:21110/23:00369938 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1088/1742-6596/2628/1/012010" target="_blank" >https://doi.org/10.1088/1742-6596/2628/1/012010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1742-6596/2628/1/012010" target="_blank" >10.1088/1742-6596/2628/1/012010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Viability of yeasts after exposure to flax-fiber based composite materials

Popis výsledku v původním jazyce

In the present study, the prepared bio-composite materials based on flax fibers containing caffeine or nano-copper (Cu) or corundum (Al2O3) as a potential biocidal substancewere prepared and tested on their toxicity. Two viability tests were performed. Firstly, thescreening test based on colouring by Methyl-blue was applied on yeast cells of strainSaccharomyces cerevisae. The number of cells density was observed under microscope andMethyl-blue was then added. Part of the cells coloured in blue and it indicated their death. Forthis reason, MTT viability assay was performed. The method is based on production of blueproduct formazan by yeasts after their metabolization of added initial substrate, 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyltetrazolium bromide. The intensity of blue-coloured formazan was measured spectrophotometrically at 485 nm. The results indicated that the inhibition ofyeasts was increased in the following rate: cu-samples (14-16 %) < flax sample without biocidal substances (21%) = pure flax (23 %) < caffeine-samples (up to 29 %) < corundum-samples (up to 35%).

Název v anglickém jazyce

Viability of yeasts after exposure to flax-fiber based composite materials

Popis výsledku anglicky

In the present study, the prepared bio-composite materials based on flax fibers containing caffeine or nano-copper (Cu) or corundum (Al2O3) as a potential biocidal substancewere prepared and tested on their toxicity. Two viability tests were performed. Firstly, thescreening test based on colouring by Methyl-blue was applied on yeast cells of strainSaccharomyces cerevisae. The number of cells density was observed under microscope andMethyl-blue was then added. Part of the cells coloured in blue and it indicated their death. Forthis reason, MTT viability assay was performed. The method is based on production of blueproduct formazan by yeasts after their metabolization of added initial substrate, 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyltetrazolium bromide. The intensity of blue-coloured formazan was measured spectrophotometrically at 485 nm. The results indicated that the inhibition ofyeasts was increased in the following rate: cu-samples (14-16 %) < flax sample without biocidal substances (21%) = pure flax (23 %) < caffeine-samples (up to 29 %) < corundum-samples (up to 35%).

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20101 - Civil engineering

Projekt

<a href="/cs/project/FW03010422" target="_blank" >FW03010422: Opatření pro snižování mikrobiálního znečištění vnitřního prostředí nízkoenergetických a pasivních obytných budov</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Journal of Physics: Conference Series 2628 (2023)

ISBN

—

ISSN

1742-6588

e-ISSN

1742-6596

Počet stran výsledku

6

Strana od-do

—

Název nakladatele

IOP Publishing Ltd.

Místo vydání

Bristol

Místo konání akce

Poprad

Datum konání akce

6. 9. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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