Fotobioreaktor pro přesnou kultivaci fotoautotrofních organismů a analýzu obsahu roztoku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F08%3A02144066" target="_blank" >RIV/68407700:21220/08:02144066 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12640/08:00009352 RIV/67179843:_____/08:00357963 RIV/60646594:_____/08:#0000031

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics

Popis výsledku v původním jazyce

Small-scale bioreactors for cultivation of photoautotrophic microbes are required for precise characterization of growth parameters of wild-type as well as engineered strains, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor allowing accurate setting of culture irradiance, temperature, and gas composition that is combined with real-time monitoring by built-in fluorometer and densitometer. The high-power light source based on light emitting diodes generated precise irradiance levels that were programmed via user-designed protocols. The irradiance, temperature, and gas composition can be static or dynamically modulated while optical density and pH can be stabilized inchemostat and turbidostat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, e.g., by sudden dilution or by circadian rhythms.

Název v anglickém jazyce

A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics

Popis výsledku anglicky

Small-scale bioreactors for cultivation of photoautotrophic microbes are required for precise characterization of growth parameters of wild-type as well as engineered strains, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor allowing accurate setting of culture irradiance, temperature, and gas composition that is combined with real-time monitoring by built-in fluorometer and densitometer. The high-power light source based on light emitting diodes generated precise irradiance levels that were programmed via user-designed protocols. The irradiance, temperature, and gas composition can be static or dynamically modulated while optical density and pH can be stabilized inchemostat and turbidostat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, e.g., by sudden dilution or by circadian rhythms.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology & Bioengineering

ISSN

0006-3592

e-ISSN

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Svazek periodika

100

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000257821800007

EID výsledku v databázi Scopus

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