Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F21%3A00350163" target="_blank" >RIV/68407700:21220/21:00350163 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68407700:21230/21:00350163 RIV/60460709:41310/20:85808 RIV/68378041:_____/21:00560412
Výsledek na webu
<a href="https://doi.org/10.37904/nanocon.2020.3763" target="_blank" >https://doi.org/10.37904/nanocon.2020.3763</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3763" target="_blank" >10.37904/nanocon.2020.3763</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline
Popis výsledku v původním jazyce
Motor vehicle emissions substantially contribute to air pollution worldwide and cause serious health problems. While the deleterious effects of diesel exhaust particulate matter (PM) have been widely studied, much less attention is paid to toxicity of PM emitted by gasoline engines although they also produce considerable amount of PM. The primary objective of this research was to assess toxic potencies of exhaust PM released by conventional gasoline engine fueled with neat gasoline (E0) or gasoline-ethanol blend (15% ethanol, v/v, E15). Despite a similar particle mass (μg PM/kg fuel) produced by both fuels, PM emitted by E15 contained higher amount of harmful polycyclic aromatic hydrocarbons (PAH) as suggested by chemical analysis. To examine the toxicity of organic PM constituents, human lung BEAS-2B cells were exposed for 4h and 24h to a subtoxic dose of E0 and E15 PM organic extracts. We used genome scale transcriptomic analysis to characterize the toxic response and to identify modulated biological process and pathways. Whereas 4h exposure to both PM extracts resulted in modulation of similar genes and pathways related to lipid and steroid metabolism, activation of PPARa, oxidative stress and immune response, 24h exposure was more specific for each extract; although both induced expression of PAH-metabolic enzymes, modulated metabolism of lipids or activated PPARa, E15 additionally deregulated variety of other pathways. Overall, the PM mass produced by both fuels was similar, however, higher PAH content in E15 PM organic extract may have contributed to more extensive toxic response particularly after 24h exposure in BEAS-2B cells.
Název v anglickém jazyce
Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline
Popis výsledku anglicky
Motor vehicle emissions substantially contribute to air pollution worldwide and cause serious health problems. While the deleterious effects of diesel exhaust particulate matter (PM) have been widely studied, much less attention is paid to toxicity of PM emitted by gasoline engines although they also produce considerable amount of PM. The primary objective of this research was to assess toxic potencies of exhaust PM released by conventional gasoline engine fueled with neat gasoline (E0) or gasoline-ethanol blend (15% ethanol, v/v, E15). Despite a similar particle mass (μg PM/kg fuel) produced by both fuels, PM emitted by E15 contained higher amount of harmful polycyclic aromatic hydrocarbons (PAH) as suggested by chemical analysis. To examine the toxicity of organic PM constituents, human lung BEAS-2B cells were exposed for 4h and 24h to a subtoxic dose of E0 and E15 PM organic extracts. We used genome scale transcriptomic analysis to characterize the toxic response and to identify modulated biological process and pathways. Whereas 4h exposure to both PM extracts resulted in modulation of similar genes and pathways related to lipid and steroid metabolism, activation of PPARa, oxidative stress and immune response, 24h exposure was more specific for each extract; although both induced expression of PAH-metabolic enzymes, modulated metabolism of lipids or activated PPARa, E15 additionally deregulated variety of other pathways. Overall, the PM mass produced by both fuels was similar, however, higher PAH content in E15 PM organic extract may have contributed to more extensive toxic response particularly after 24h exposure in BEAS-2B cells.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
30108 - Toxicology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
NANOCON Conference Proceedings - International Conference on Nanomaterials
ISBN
978-80-87294-98-7
ISSN
—
e-ISSN
2694-930X
Počet stran výsledku
6
Strana od-do
453-458
Název nakladatele
TANGER
Místo vydání
Ostrava
Místo konání akce
Brno
Datum konání akce
21. 10. 2020
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000664505500077