Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F15%3A00225051" target="_blank" >RIV/68407700:21230/15:00225051 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/15:00457605 RIV/67985882:_____/15:00457605

Výsledek na webu

<a href="http://dx.doi.org/10.1117/12.2069897" target="_blank" >http://dx.doi.org/10.1117/12.2069897</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2069897" target="_blank" >10.1117/12.2069897</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells

Popis výsledku v původním jazyce

Optical spectral analysis of the ultra-weak photon emission (UPE) could be utilized for non-invasive diagnostic of state of biological systems and for elucidation of underlying mechanisms of UPE generation. Optical spectra of UPE from differentiated HL-60 cells and yeast cells (Saccharomyces cerevisiae) were investigated. Induced photon emission of neutrophil-like cells and spontaneous photon emission of yeast cells were measured using highly sensitive photomultiplier module Hamamatsu H7360-01 in a thermally regulated light-tight chamber. The respiratory burst of neutrophil-like HL-60 cells was induced with the PMA (phorbol 12-myristate, 13-acetate). PMA activates an assembly of NADPH oxidase, which induces a rapid formation of reactive oxygen species(ROS). Long-pass edge filters (wavelength 350, from 400 to 600 with 25 nm resolution and 650 nm) were used for optical spectral analysis. Propagation of error of indirect measurements and standard deviation were used to assess reliability

Název v anglickém jazyce

Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells

Popis výsledku anglicky

Optical spectral analysis of the ultra-weak photon emission (UPE) could be utilized for non-invasive diagnostic of state of biological systems and for elucidation of underlying mechanisms of UPE generation. Optical spectra of UPE from differentiated HL-60 cells and yeast cells (Saccharomyces cerevisiae) were investigated. Induced photon emission of neutrophil-like cells and spontaneous photon emission of yeast cells were measured using highly sensitive photomultiplier module Hamamatsu H7360-01 in a thermally regulated light-tight chamber. The respiratory burst of neutrophil-like HL-60 cells was induced with the PMA (phorbol 12-myristate, 13-acetate). PMA activates an assembly of NADPH oxidase, which induces a rapid formation of reactive oxygen species(ROS). Long-pass edge filters (wavelength 350, from 400 to 600 with 25 nm resolution and 650 nm) were used for optical spectral analysis. Propagation of error of indirect measurements and standard deviation were used to assess reliability

Druh

D - Stať ve sborníku

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-29294S" target="_blank" >GA13-29294S: Fotonické biosignály: měření a charakterizace</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proc. SPIE 9450, Photonics, Devices, and Systems VI

ISBN

978-1-62841-566-7

ISSN

0277-786X

e-ISSN

—

Počet stran výsledku

7

Strana od-do

"94500O-1"-"94500O-7"

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

Prague

Datum konání akce

27. 8. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000349404500023