Effect of dose and dose rate of gamma radiation on catalytic activity of catalase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F10%3A00176318" target="_blank" >RIV/68407700:21340/10:00176318 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of dose and dose rate of gamma radiation on catalytic activity of catalase

Popis výsledku v původním jazyce

Catalytic activity of gamma irradiated catalase from bovine liver was studied for hydrogen peroxide decomposition at constant temperature and pressure. The measurement was performed at temperatures 27, 32, 37, 42 and 47 C. Solutions containing 1 and 0.01g dm-3 of catalase in phosphate buffer were used for the study. Repeatability of both sample preparation and kinetics measurement was experimentally verified. Rate constants of the reaction were determined for all temperatures and the activation energywas evaluated from Arrhenius plot. Gamma irradiation was performed using radionuclide source Gammacell 220 at two different dose rates 5.5 and 70 Gy h-1, with doses ranging from 10 to 1000 Gy. The observed reaction of irradiated and non-irradiated catalase with hydrogen peroxide is of the first order. Irradiation significantly decreases catalytic activity of catalase, but the activation energy does not depend on the dose.

Název v anglickém jazyce

Effect of dose and dose rate of gamma radiation on catalytic activity of catalase

Popis výsledku anglicky

Catalytic activity of gamma irradiated catalase from bovine liver was studied for hydrogen peroxide decomposition at constant temperature and pressure. The measurement was performed at temperatures 27, 32, 37, 42 and 47 C. Solutions containing 1 and 0.01g dm-3 of catalase in phosphate buffer were used for the study. Repeatability of both sample preparation and kinetics measurement was experimentally verified. Rate constants of the reaction were determined for all temperatures and the activation energywas evaluated from Arrhenius plot. Gamma irradiation was performed using radionuclide source Gammacell 220 at two different dose rates 5.5 and 70 Gy h-1, with doses ranging from 10 to 1000 Gy. The observed reaction of irradiated and non-irradiated catalase with hydrogen peroxide is of the first order. Irradiation significantly decreases catalytic activity of catalase, but the activation energy does not depend on the dose.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CH - Jaderná a kvantová chemie, fotochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Radioanalytical and Nuclear Chemistry

ISSN

0236-5731

e-ISSN

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Svazek periodika

286

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000285099700004

EID výsledku v databázi Scopus

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