Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F15%3A00369333" target="_blank" >RIV/68407700:21340/15:00369333 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/15:00456882

Výsledek na webu

<a href="https://doi.org/10.1016/j.jcis.2015.04.007" target="_blank" >https://doi.org/10.1016/j.jcis.2015.04.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcis.2015.04.007" target="_blank" >10.1016/j.jcis.2015.04.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters

Popis výsledku v původním jazyce

Polyurethene (PU) catheters were coated with 3-(trimethoxysilyl)-propyldimethyloctadecylammonium chloride (QAS) by means of a multistep process which involved a vapor phase plasma-induced graft-polymerization of acrylic acid (AAc). The AAc coating, whose stability in aqueous media was assessed by immersion in Phospate Buffer Saline (PBS), was characterized by means of Attenuated Total Reflectance Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, the COOH surface density was evaluated by a colorimetric assay with Methylene Blue. Carrying a negative charge at neutral pH, AAc coatings were proficient in positively charged molecules (like QAS) adsorption. ATR/FTIR spectroscopy and a colorimetric assay with Bromophenol Blue allowed us to verify the presence and the uniformity of the QAS coating on the PU catheters and the positive effect of the AAc graft-polymerization on the QAS adsorption. Morphological characterization of the QAS-modified catheters was performed by means of Atomic Force Microscopy (AFM). QAS-coated catheters displayed in vitro antimicrobial activity against Gram-negative Escherichia coli bacterial cells.

Název v anglickém jazyce

Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters

Popis výsledku anglicky

Polyurethene (PU) catheters were coated with 3-(trimethoxysilyl)-propyldimethyloctadecylammonium chloride (QAS) by means of a multistep process which involved a vapor phase plasma-induced graft-polymerization of acrylic acid (AAc). The AAc coating, whose stability in aqueous media was assessed by immersion in Phospate Buffer Saline (PBS), was characterized by means of Attenuated Total Reflectance Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, the COOH surface density was evaluated by a colorimetric assay with Methylene Blue. Carrying a negative charge at neutral pH, AAc coatings were proficient in positively charged molecules (like QAS) adsorption. ATR/FTIR spectroscopy and a colorimetric assay with Bromophenol Blue allowed us to verify the presence and the uniformity of the QAS coating on the PU catheters and the positive effect of the AAc graft-polymerization on the QAS adsorption. Morphological characterization of the QAS-modified catheters was performed by means of Atomic Force Microscopy (AFM). QAS-coated catheters displayed in vitro antimicrobial activity against Gram-negative Escherichia coli bacterial cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Colloid and Interface Science

ISSN

0021-9797

e-ISSN

1095-7103

Svazek periodika

451

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

78-84

Kód UT WoS článku

000354150900010

EID výsledku v databázi Scopus

2-s2.0-84928752788