Non-Fouling Biodegradable Poly(epsilon-caprolactone) Nanofibers for Tissue Engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F16%3A00310400" target="_blank" >RIV/68407700:21340/16:00310400 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/16:00454747 RIV/61389013:_____/16:00454747

Výsledek na webu

<a href="https://doi.org/10.1002/mabi.201500252" target="_blank" >https://doi.org/10.1002/mabi.201500252</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mabi.201500252" target="_blank" >10.1002/mabi.201500252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Non-Fouling Biodegradable Poly(epsilon-caprolactone) Nanofibers for Tissue Engineering

Popis výsledku v původním jazyce

Poly(ε-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell-matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion. Four antifouling polymer brushes are grown from biodegradable poly(ε-caprolactone) nanofibers without impairing their unique architecture. The nanofibers are challenged with mouse embryonic fibroblasts. Fibroblasts are repelled from the nanofibers coated with brushes while the cells could rapidly adhere and form cell-matrix adhesions on pristine nanofibers. The nanofibers modified with protein repellent brushes are able to suppress cell adhesion.

Název v anglickém jazyce

Non-Fouling Biodegradable Poly(epsilon-caprolactone) Nanofibers for Tissue Engineering

Popis výsledku anglicky

Poly(ε-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell-matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion. Four antifouling polymer brushes are grown from biodegradable poly(ε-caprolactone) nanofibers without impairing their unique architecture. The nanofibers are challenged with mouse embryonic fibroblasts. Fibroblasts are repelled from the nanofibers coated with brushes while the cells could rapidly adhere and form cell-matrix adhesions on pristine nanofibers. The nanofibers modified with protein repellent brushes are able to suppress cell adhesion.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10302 - Condensed matter physics (including formerly solid state physics, supercond.)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macromolecular Bioscience

ISSN

1616-5187

e-ISSN

1616-5195

Svazek periodika

16

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

83-94

Kód UT WoS článku

000368642300008

EID výsledku v databázi Scopus

2-s2.0-84954290374