Surface modification of 99mTC-HAp-NPs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F18%3A00327227" target="_blank" >RIV/68407700:21340/18:00327227 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Surface modification of 99mTC-HAp-NPs

Popis výsledku v původním jazyce

Hydroxyapatite (HAp) is one the main mineral bones and teeth components and biocompatible material used in orthopaedic implants. It might be used as a drug carrier in diagnostics and treatment of cancer. The sufficient in vivo stability without fast aggregation of the Hap-NPs is required for theranostic application. Various phosphonic acids were studied as potential HAp stabilisers and technetium-99m chelator. Hydroxyapatite nanoparticles were prepared by precipitation of Ca(NO3)2 with (NH4)2HPO4 at pH=11. The precipitate was washed, lyophilized and crushed. Stabilized samples were prepared from already-made HAp-NPs by ultrasound dispergation in corresponding phosphonic acid solution in water (1 mg/ml). The hydrodynamic size distributions of studied stabilized particles were determined using dynamic light scattering (Zetasizer, Malvern, UK). Samples were labelled with technetium-99m eluted from 99Mo/99mTc generator (DRYTEC, GE Healthcare). The labelling yield ranged to about 90%. Subsequent in vitro stability studies were carried out in bovine serum, bovine plasma, saline and 5% albumin solution. Measurements of released activity revealed that samples exhibit the highest stability in saline (released activity of about 10%). The lowest stability was shown to be in blood plasma (released activity of about 20%).

Název v anglickém jazyce

Surface modification of 99mTC-HAp-NPs

Popis výsledku anglicky

Hydroxyapatite (HAp) is one the main mineral bones and teeth components and biocompatible material used in orthopaedic implants. It might be used as a drug carrier in diagnostics and treatment of cancer. The sufficient in vivo stability without fast aggregation of the Hap-NPs is required for theranostic application. Various phosphonic acids were studied as potential HAp stabilisers and technetium-99m chelator. Hydroxyapatite nanoparticles were prepared by precipitation of Ca(NO3)2 with (NH4)2HPO4 at pH=11. The precipitate was washed, lyophilized and crushed. Stabilized samples were prepared from already-made HAp-NPs by ultrasound dispergation in corresponding phosphonic acid solution in water (1 mg/ml). The hydrodynamic size distributions of studied stabilized particles were determined using dynamic light scattering (Zetasizer, Malvern, UK). Samples were labelled with technetium-99m eluted from 99Mo/99mTc generator (DRYTEC, GE Healthcare). The labelling yield ranged to about 90%. Subsequent in vitro stability studies were carried out in bovine serum, bovine plasma, saline and 5% albumin solution. Measurements of released activity revealed that samples exhibit the highest stability in saline (released activity of about 10%). The lowest stability was shown to be in blood plasma (released activity of about 20%).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

<a href="/cs/project/NV16-30544A" target="_blank" >NV16-30544A: Nová vícefázová nanodiagnostika pro zobrazování nádorových onemocnění a predikci efektivity antiangiogenní terapie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů