A new macro-imager based on Tpx3Cam optical camera for PLIM applications
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F20%3A00343509" target="_blank" >RIV/68407700:21340/20:00343509 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1117/12.2555387" target="_blank" >http://dx.doi.org/10.1117/12.2555387</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2555387" target="_blank" >10.1117/12.2555387</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A new macro-imager based on Tpx3Cam optical camera for PLIM applications
Popis výsledku v původním jazyce
The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.
Název v anglickém jazyce
A new macro-imager based on Tpx3Cam optical camera for PLIM applications
Popis výsledku anglicky
The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
10300 - Physical sciences
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceedings of SPIE Photonics West, OPTO, 2020
ISBN
978-1-5106-3378-0
ISSN
0277-786X
e-ISSN
—
Počet stran výsledku
8
Strana od-do
1-8
Název nakladatele
SPIE
Místo vydání
Washington
Místo konání akce
San Francisco, California
Datum konání akce
1. 2. 2020
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000674749600032