Eukaryots in human digestive tract
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F11%3A00192484" target="_blank" >RIV/68407700:21460/11:00192484 - isvavai.cz</a>
Výsledek na webu
<a href="http://imbm.fbmi.cvut.cz/soubory/sbornik.pdf" target="_blank" >http://imbm.fbmi.cvut.cz/soubory/sbornik.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Eukaryots in human digestive tract
Popis výsledku v původním jazyce
The aim of work was detection of micro-eukaryota in faecal samples from healthy people and people suffering from celiac disease and comparison of the results. DNA was isolated from faecal samples of 20 people. It was analyzed using the PCR-DGGE method focused on 18S rRNA genes and PCR focused on fungal ITS regions, both followed by sequencing, and it was tested for presence of 4 bacterial genera (Aspergillus, Candida, Saccharomyces and Penicillium) using real-time PCR. Sequences were compared with database of nucleotide sequences for generic designation. DGGE profiles showed high diversity and low inter individual similarity of the intestinal micro-eukaryota. The sequence analysis revealed presence of Blastocystis sp., Candida albicans and Malassezia restricta, each in one subject. Most of the sequences were similar to different nonspecified sequences from human gut metagenome DNA. Real time PCR detected Saccharomyces cerevisiae in five subjects. No significant differences were found b
Název v anglickém jazyce
Eukaryots in human digestive tract
Popis výsledku anglicky
The aim of work was detection of micro-eukaryota in faecal samples from healthy people and people suffering from celiac disease and comparison of the results. DNA was isolated from faecal samples of 20 people. It was analyzed using the PCR-DGGE method focused on 18S rRNA genes and PCR focused on fungal ITS regions, both followed by sequencing, and it was tested for presence of 4 bacterial genera (Aspergillus, Candida, Saccharomyces and Penicillium) using real-time PCR. Sequences were compared with database of nucleotide sequences for generic designation. DGGE profiles showed high diversity and low inter individual similarity of the intestinal micro-eukaryota. The sequence analysis revealed presence of Blastocystis sp., Candida albicans and Malassezia restricta, each in one subject. Most of the sequences were similar to different nonspecified sequences from human gut metagenome DNA. Real time PCR detected Saccharomyces cerevisiae in five subjects. No significant differences were found b
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů