Using genetic engineering methods for detection lower fungi in digestive tract of primates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202752" target="_blank" >RIV/68407700:21460/12:00202752 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Using genetic engineering methods for detection lower fungi in digestive tract of primates

Popis výsledku v původním jazyce

The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future.

Název v anglickém jazyce

Using genetic engineering methods for detection lower fungi in digestive tract of primates

Popis výsledku anglicky

The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instuments and Methods for Biology and Medicine 2012

ISBN

978-80-01-05119-1

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

33-35

Název nakladatele

Czech Technical University

Místo vydání

Prague

Místo konání akce

Kladno

Datum konání akce

29. 5. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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