Bonding and bio-properties of hybrid laser/magnetron Cr-enriched

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F16%3A00232362" target="_blank" >RIV/68407700:21460/16:00232362 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2015.09.006" target="_blank" >http://dx.doi.org/10.1016/j.msec.2015.09.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2015.09.006" target="_blank" >10.1016/j.msec.2015.09.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bonding and bio-properties of hybrid laser/magnetron Cr-enriched

Popis výsledku v původním jazyce

Chromium-enriched diamond-like carbon (DLC) layers were prepared by a hybrid technology using a combination of pulsed laser deposition (PLD) and magnetron sputtering. XRD revealed no chromium peaks, indicating that the layers are mostly amorphous. Carbon (sp2 and sp3 bonds) and chromium bonds were determined by XPS from C 1s, O 1s, and Cr 2p photoelectron peaks. Depending on the deposition conditions, the concentration of Cr in DLC layers moved from zero to 10 at.% for as-received sample surfaces, and to about 31 at.% after mild sputter-cleaning by argon ion cluster beam. It should be noted that the most stable Cr3 + bonding state is in Cr2O3 and Cr(OH)3, and that there is the toxic Cr6 + state in CrO3. The surface content of hexavalent chromium in the Cr 2p3/2 spectra is rather low, but discernible. The population density of Saos-2 cells was the highest in samples containing higher concentrations of chromium 7.7 and 10 at.%. This means that higher concentrations of chromium supported the cell adhesion and proliferation. In addition, as revealed by a LIVE/DEAD viability/cytotoxicity kit, the cells on all Cr-containing samples maintained high viability (96 to 99%) on days 1 and 3 after seeding. However, this seemingly positive cell behavior could be associated with the risk of dedifferentiation and oncogenic transformation of cells.

Název v anglickém jazyce

Bonding and bio-properties of hybrid laser/magnetron Cr-enriched

Popis výsledku anglicky

Chromium-enriched diamond-like carbon (DLC) layers were prepared by a hybrid technology using a combination of pulsed laser deposition (PLD) and magnetron sputtering. XRD revealed no chromium peaks, indicating that the layers are mostly amorphous. Carbon (sp2 and sp3 bonds) and chromium bonds were determined by XPS from C 1s, O 1s, and Cr 2p photoelectron peaks. Depending on the deposition conditions, the concentration of Cr in DLC layers moved from zero to 10 at.% for as-received sample surfaces, and to about 31 at.% after mild sputter-cleaning by argon ion cluster beam. It should be noted that the most stable Cr3 + bonding state is in Cr2O3 and Cr(OH)3, and that there is the toxic Cr6 + state in CrO3. The surface content of hexavalent chromium in the Cr 2p3/2 spectra is rather low, but discernible. The population density of Saos-2 cells was the highest in samples containing higher concentrations of chromium 7.7 and 10 at.%. This means that higher concentrations of chromium supported the cell adhesion and proliferation. In addition, as revealed by a LIVE/DEAD viability/cytotoxicity kit, the cells on all Cr-containing samples maintained high viability (96 to 99%) on days 1 and 3 after seeding. However, this seemingly positive cell behavior could be associated with the risk of dedifferentiation and oncogenic transformation of cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science and Engineering C, Biomimetic and Supramolecular Systems

ISSN

0928-4931

e-ISSN

—

Svazek periodika

58

Číslo periodika v rámci svazku

58

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

1217-1224

Kód UT WoS článku

000364247500145

EID výsledku v databázi Scopus

2-s2.0-84943563493