Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F17%3A00313968" target="_blank" >RIV/68407700:21460/17:00313968 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy

Popis výsledku v původním jazyce

Physical stability of model biomembranes in form of vesicles is being investigated since vesicles become a tool for simulations and research of biomembranes and their interactions with the environment or other particles. In this study, we work with small unilamellar vesicles (SUV) composed of phospholipids POPC and DOPS in different ratios and we optimized their preparation protocol. Physical stability of model biomembranes as vesicles can be described as resistance to aggregation and biomembrane disruption. We used fluorescence correlation spectroscopy (FCS) and fluorescence lifetime correlation spectroscopy (FLCS) as suitable techniques to detect these phenomena by observing the diffusion of the fluorescent labels in vesicles. Stability of vesicles was investigated in two steps - first with SUVs labelled by only one dye and next with double-labelled vesicles. Obtained results showed that the stability is dependent on the composition of the vesicles and by adjusting the POPC:DOPS ratio we can achieve up to 6 day stability.

Název v anglickém jazyce

Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy

Popis výsledku anglicky

Physical stability of model biomembranes in form of vesicles is being investigated since vesicles become a tool for simulations and research of biomembranes and their interactions with the environment or other particles. In this study, we work with small unilamellar vesicles (SUV) composed of phospholipids POPC and DOPS in different ratios and we optimized their preparation protocol. Physical stability of model biomembranes as vesicles can be described as resistance to aggregation and biomembrane disruption. We used fluorescence correlation spectroscopy (FCS) and fluorescence lifetime correlation spectroscopy (FLCS) as suitable techniques to detect these phenomena by observing the diffusion of the fluorescent labels in vesicles. Stability of vesicles was investigated in two steps - first with SUVs labelled by only one dye and next with double-labelled vesicles. Obtained results showed that the stability is dependent on the composition of the vesicles and by adjusting the POPC:DOPS ratio we can achieve up to 6 day stability.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instruments and Methods for Biology and Medicine 2017

ISBN

978-80-01-06303-3

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

1-5

Název nakladatele

Česká technika - nakladatelství ČVUT, ČVUT v Praze

Místo vydání

Praha

Místo konání akce

Kladno

Datum konání akce

5. 5. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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