Estimation of intestine microbiome in mice obesity model using tools of molecular genetics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00330789" target="_blank" >RIV/68407700:21460/18:00330789 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of intestine microbiome in mice obesity model using tools of molecular genetics

Popis výsledku v původním jazyce

Obesity and its associated metabolic disorders are characterized by specific changes in the structure and function of an intestinal microbe which is actively involved in many of the host’s physiological processes. A change in the intestinal microbial composition along with its genetic predispositions can contribute to metabolic disorders in the host. The next generation sequencing method on the Ion Torrent platform was used for bacterial diversity analyses, to compare the digestive tract microbes of obese and control mouse models (C57BL / 6N males) under various nutritional conditions. Differences in the diversity of caecum and colon microbiota were analyzed, depending on the type of diet, and phenotypic differences of mouse models were also observed. The high-fat diet compared to the standard diet for the microbiomes of both tracts increased the Firmicutes to Bacteroidetes ratio, food supplements (omega-3 acids) had tendencies to shift the microbial composition back towards to the STD diet, more significant changes were observed in the caecum. There were differences in the representation of the Clostridiales and Bacteroides bacteria, for example, the family S24-7, Lachnospiracae or Prevotellaceae.

Název v anglickém jazyce

Estimation of intestine microbiome in mice obesity model using tools of molecular genetics

Popis výsledku anglicky

Obesity and its associated metabolic disorders are characterized by specific changes in the structure and function of an intestinal microbe which is actively involved in many of the host’s physiological processes. A change in the intestinal microbial composition along with its genetic predispositions can contribute to metabolic disorders in the host. The next generation sequencing method on the Ion Torrent platform was used for bacterial diversity analyses, to compare the digestive tract microbes of obese and control mouse models (C57BL / 6N males) under various nutritional conditions. Differences in the diversity of caecum and colon microbiota were analyzed, depending on the type of diet, and phenotypic differences of mouse models were also observed. The high-fat diet compared to the standard diet for the microbiomes of both tracts increased the Firmicutes to Bacteroidetes ratio, food supplements (omega-3 acids) had tendencies to shift the microbial composition back towards to the STD diet, more significant changes were observed in the caecum. There were differences in the representation of the Clostridiales and Bacteroides bacteria, for example, the family S24-7, Lachnospiracae or Prevotellaceae.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů