Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F09%3A%230000187" target="_blank" >RIV/70565813:_____/09:#0000187 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Popis výsledku v původním jazyce

This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe wasdeveloped for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative assay of copy numbers of the target gene. Avian influenza virus A/Cygnus olor/Brno-cz/2006 was adapted to grow in VERO cells. In the same passage of cell cultivation, the concentration of viral RNA was determined to be 1.01 x 10(7) copies/ml and TCID50 was 10(4.2)/ml. From thesevalues the ratio of one RNA copy to 0.00157 virion capable of VERO cells infection was calculated. This ratio was used to estimate the virus concentrations in the tissues of dead mute swans

Název v anglickém jazyce

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Popis výsledku anglicky

This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe wasdeveloped for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative assay of copy numbers of the target gene. Avian influenza virus A/Cygnus olor/Brno-cz/2006 was adapted to grow in VERO cells. In the same passage of cell cultivation, the concentration of viral RNA was determined to be 1.01 x 10(7) copies/ml and TCID50 was 10(4.2)/ml. From thesevalues the ratio of one RNA copy to 0.00157 virion capable of VERO cells infection was calculated. This ratio was used to estimate the virus concentrations in the tissues of dead mute swans

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VETERINARNI MEDICINA

ISSN

0375-8427

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000272392600007

EID výsledku v databázi Scopus

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