Tick-borne encephalitis in dogs: application of "nested real-time RT-PCR" for intravital virus detection.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F15%3A%230000411" target="_blank" >RIV/70565813:_____/15:#0000411 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.researchgate.net/journal/0005-9366_Berliner_und_Muenchener_tieraerztliche_Wochenschrift/2" target="_blank" >https://www.researchgate.net/journal/0005-9366_Berliner_und_Muenchener_tieraerztliche_Wochenschrift/2</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tick-borne encephalitis in dogs: application of "nested real-time RT-PCR" for intravital virus detection.

Popis výsledku v původním jazyce

Tick-borne encephalitis (TBE) virus is a tick-transmitted virus causing disorders of the nervous system in humans, monkeys, dogs and horses (rarely). At present the detection of TBE infection in dogs is performed by confirmation of seroconversion in paired samples of serum in clinical practice. The intention of the study was the assessment of the possible application of nested real-time RT-PCR for detection of TBE virus in canine blood. The study was carried out in 2011-2012 using samples originating in the Czech Republic, South Moravian region (region with endemic occurrence of TBE). The dogs were randomly selected from the patients visiting the clinic during this time period. Of the total amount of 159 canine blood samples, 20 samples were tested with a PCR-positive result (12.6%). Out of these 20 animals, the neurological clinical symptoms typical of TBE were detected in seven dogs. PCR-positive results were found between March and November. Three dogs were tested with a competitive ELISA-positive result and a "nested real-time RT-PCR"-positive result concurrently. In the group of 159 dogs the value of seroprevalence was found to be 11.3%.

Název v anglickém jazyce

Tick-borne encephalitis in dogs: application of "nested real-time RT-PCR" for intravital virus detection.

Popis výsledku anglicky

Tick-borne encephalitis (TBE) virus is a tick-transmitted virus causing disorders of the nervous system in humans, monkeys, dogs and horses (rarely). At present the detection of TBE infection in dogs is performed by confirmation of seroconversion in paired samples of serum in clinical practice. The intention of the study was the assessment of the possible application of nested real-time RT-PCR for detection of TBE virus in canine blood. The study was carried out in 2011-2012 using samples originating in the Czech Republic, South Moravian region (region with endemic occurrence of TBE). The dogs were randomly selected from the patients visiting the clinic during this time period. Of the total amount of 159 canine blood samples, 20 samples were tested with a PCR-positive result (12.6%). Out of these 20 animals, the neurological clinical symptoms typical of TBE were detected in seven dogs. PCR-positive results were found between March and November. Three dogs were tested with a competitive ELISA-positive result and a "nested real-time RT-PCR"-positive result concurrently. In the group of 159 dogs the value of seroprevalence was found to be 11.3%.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/VF20112015013" target="_blank" >VF20112015013: Výzkum moderních metod detekce a identifikace nebezpečných CBRN látek a materiálů, metod snížení jejich nebezpečnosti a dekontaminace; výzkum moderních prostředků ochrany osob a prvků kritické infrastruktury</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Berliner und Munchener tierarztliche Wochenschrift

ISSN

0005-9366

e-ISSN

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Svazek periodika

128

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

397-400

Kód UT WoS článku

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EID výsledku v databázi Scopus

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