Use of the HRM Analysis in Pathogen Identification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F18%3AN0000034" target="_blank" >RIV/70565813:_____/18:N0000034 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of the HRM Analysis in Pathogen Identification

Popis výsledku v původním jazyce

The HRM – high resolution melting – analysis represents a post-PCR method enabling the differentiation according to the sequence of the amplicon. Thus, the amplicon is analyzed right after the real-time PCR in the same machine without further manipulation with samples. The so-called third generation intercalating dyes are used in the reaction, and special software for the analysis of raw data is essential. To demonstrate the usefulness of the HRM analysis, tick-borne encephalitis virus (TBEV) and Brucella sp. were chosen as examples of pathogenic biological agents. The forth involves three distinct subtypes connected with a varied course of the disease, the latter comprises a high number of genetically closely related species, although some of them possess a high risk for humans. Accordingly, the HRM analysis was selected to distinguish B. abortus, B. melitensis and B. suis from the rest of Brucella sp.

Název v anglickém jazyce

Use of the HRM Analysis in Pathogen Identification

Popis výsledku anglicky

The HRM – high resolution melting – analysis represents a post-PCR method enabling the differentiation according to the sequence of the amplicon. Thus, the amplicon is analyzed right after the real-time PCR in the same machine without further manipulation with samples. The so-called third generation intercalating dyes are used in the reaction, and special software for the analysis of raw data is essential. To demonstrate the usefulness of the HRM analysis, tick-borne encephalitis virus (TBEV) and Brucella sp. were chosen as examples of pathogenic biological agents. The forth involves three distinct subtypes connected with a varied course of the disease, the latter comprises a high number of genetically closely related species, although some of them possess a high risk for humans. Accordingly, the HRM analysis was selected to distinguish B. abortus, B. melitensis and B. suis from the rest of Brucella sp.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/VI20172019063" target="_blank" >VI20172019063: Vývoj nových metodik pro detekci biologických agens v oblastech souvisejících s dodržováním Úmluvy o zákazu biologických zbraní</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů