Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F11%3A43865491" target="_blank" >RIV/70883521:28110/11:43865491 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/11:43865491

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-642-19038-4_16" target="_blank" >http://dx.doi.org/10.1007/978-3-642-19038-4_16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-642-19038-4_16" target="_blank" >10.1007/978-3-642-19038-4_16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment

Popis výsledku v původním jazyce

Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seededon the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surfaceroughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications.

Název v anglickém jazyce

Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment

Popis výsledku anglicky

Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seededon the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surfaceroughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JK - Koroze a povrchové úpravy materiálu

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0111" target="_blank" >ED2.1.00/03.0111: Centrum polymerních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Progress in Colloid and Polymer Science

ISSN

0340-255X

e-ISSN

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Svazek periodika

138

Číslo periodika v rámci svazku

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Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

89-94

Kód UT WoS článku

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EID výsledku v databázi Scopus

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