Isolation of 2-ethylhexanol utilizing Pseudomonas japonica strain P for potential remediation of contaminated environments

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of 2-ethylhexanol utilizing Pseudomonas japonica strain P for potential remediation of contaminated environments

Popis výsledku v původním jazyce

An isolation of 2-ethylhexanol utilizing strain for potential remediation of plasticizer(s) contaminated environments was the main aim of the work. The active degrading strain designated as EHOL P was isolated from activated sludge originated from wastewater treatment plant treating common municipal wastewater. The isolated strain was characterized and its degradation ability was examined. Biodegradation tests were performed in liquid media containing 2-ethylhexanol as the only carbon and energy source; 2-ethylhexanol was added at initial concentration of 500 mg/l and the biodegradation was monitored via 2-ethylhexanol determination by gas chromatography, after performing solid phase extraction. Moreover, concentrations of dissolved organic carbon (DOC) throughout the degradation tests were monitored as well. Identification of the strain was done by 16S rDNA sequence analysis. The strain EHOL P was revealed to be non-fermenting, catalase positive rods. The study of its growth properties showed that its optimal growth temperature, pH optimum and salt concentration suitable for its growth were 5 – 45 °C, pH 6 – 9 and 0 – 5.5 % NaCl (w/v), respectively. Subsequent identification revealed that the strain is a member of the genus Pseudomonas and closely related to P. japonica with 99% similarity. Successful biodegradation of 500 mg/l of 2-ethylhexanol occurred during 36 hours and the substrate determinations as well as DOC monitoring suggest that the strain is capable of complete 2-ethylhexanol degradation. Our work showed that bacterial strain(s) with 2-ethylhexanol degrading capability occur naturally in common activated sludge; further properties of isolated strain, including verifying anticipated mechanism of 2-ethylhexanol degradation, are being studied.

Název v anglickém jazyce

Isolation of 2-ethylhexanol utilizing Pseudomonas japonica strain P for potential remediation of contaminated environments

Popis výsledku anglicky

An isolation of 2-ethylhexanol utilizing strain for potential remediation of plasticizer(s) contaminated environments was the main aim of the work. The active degrading strain designated as EHOL P was isolated from activated sludge originated from wastewater treatment plant treating common municipal wastewater. The isolated strain was characterized and its degradation ability was examined. Biodegradation tests were performed in liquid media containing 2-ethylhexanol as the only carbon and energy source; 2-ethylhexanol was added at initial concentration of 500 mg/l and the biodegradation was monitored via 2-ethylhexanol determination by gas chromatography, after performing solid phase extraction. Moreover, concentrations of dissolved organic carbon (DOC) throughout the degradation tests were monitored as well. Identification of the strain was done by 16S rDNA sequence analysis. The strain EHOL P was revealed to be non-fermenting, catalase positive rods. The study of its growth properties showed that its optimal growth temperature, pH optimum and salt concentration suitable for its growth were 5 – 45 °C, pH 6 – 9 and 0 – 5.5 % NaCl (w/v), respectively. Subsequent identification revealed that the strain is a member of the genus Pseudomonas and closely related to P. japonica with 99% similarity. Successful biodegradation of 500 mg/l of 2-ethylhexanol occurred during 36 hours and the substrate determinations as well as DOC monitoring suggest that the strain is capable of complete 2-ethylhexanol degradation. Our work showed that bacterial strain(s) with 2-ethylhexanol degrading capability occur naturally in common activated sludge; further properties of isolated strain, including verifying anticipated mechanism of 2-ethylhexanol degradation, are being studied.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů