Calcium filled bacterial cellulose based composite hydrogel for bone tissue engineering: Optical microscopy and bone marker analysis

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/70883521:28610/19:63522501

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Calcium filled bacterial cellulose based composite hydrogel for bone tissue engineering: Optical microscopy and bone marker analysis

Popis výsledku v původním jazyce

Bone related disorders affect 75 million individual through elaborating significant bone fracture situation throughout Europe, USA and Japan. The possible treatment methods are notably expensive. The hydrogel based bioactive composite scaffold can be a suitable approach due to its cost-effective production attributes. The primary focus of the work is the biological characterization (i.e. cell growth study after 5 days of incubation with sample through optical microscopy and bone marker analysis or alkaline phosphate expression study) of the novel inorganic calcium filled bacterial cellulose (BC) based hydrogel composite scaffolds in the context of their application in bone tissue engineering application. BC based hydrogel scaffolds were developed by applying the homogeneous suspension of BC (holding 99% water) with carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP). β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) were incorporated to achieve the calcium phosphate filled hydrogel scaffolds (termed as “BC-PVP-β-TCP/HA” and “BC-CMC-β-TCP/HA”). These hydrogel scaffolds were in vitro bio-mineralized to incorporate CaCO3 (termed as “BC-PVP-β-TCP/HA-CaCO3” and “BC-CMC-β-TCP/HA-CaCO3”). BC-PVP and BC-CMC hydrogel scaffolds were used as control set. Human diploid fibroblast cells, Lep-3 and mouse bone explant cell line (BEC) were used in our study. The cell viability was found significant for BC-PVP-β-TCP/HA with both cell lines. The ALP expression in BEC cell culture of BC-PVP-β-TCP/HA and BC-CMC-β-TCP/HA-CaCO3 hydrogel scaffold is also found significant. These results suggest the BC-PVP-β-TCP/HA hydrogel composite scaffold has the notable potentiality for the soft bone tissue engineering application.

Název v anglickém jazyce

Calcium filled bacterial cellulose based composite hydrogel for bone tissue engineering: Optical microscopy and bone marker analysis

Popis výsledku anglicky

Bone related disorders affect 75 million individual through elaborating significant bone fracture situation throughout Europe, USA and Japan. The possible treatment methods are notably expensive. The hydrogel based bioactive composite scaffold can be a suitable approach due to its cost-effective production attributes. The primary focus of the work is the biological characterization (i.e. cell growth study after 5 days of incubation with sample through optical microscopy and bone marker analysis or alkaline phosphate expression study) of the novel inorganic calcium filled bacterial cellulose (BC) based hydrogel composite scaffolds in the context of their application in bone tissue engineering application. BC based hydrogel scaffolds were developed by applying the homogeneous suspension of BC (holding 99% water) with carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP). β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) were incorporated to achieve the calcium phosphate filled hydrogel scaffolds (termed as “BC-PVP-β-TCP/HA” and “BC-CMC-β-TCP/HA”). These hydrogel scaffolds were in vitro bio-mineralized to incorporate CaCO3 (termed as “BC-PVP-β-TCP/HA-CaCO3” and “BC-CMC-β-TCP/HA-CaCO3”). BC-PVP and BC-CMC hydrogel scaffolds were used as control set. Human diploid fibroblast cells, Lep-3 and mouse bone explant cell line (BEC) were used in our study. The cell viability was found significant for BC-PVP-β-TCP/HA with both cell lines. The ALP expression in BEC cell culture of BC-PVP-β-TCP/HA and BC-CMC-β-TCP/HA-CaCO3 hydrogel scaffold is also found significant. These results suggest the BC-PVP-β-TCP/HA hydrogel composite scaffold has the notable potentiality for the soft bone tissue engineering application.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

<a href="/cs/project/LO1504" target="_blank" >LO1504: Centrum polymerních systémů plus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů