Isotopic and microbiological evidence of microbial methane and CO2 in the Upper Silesian Coal Basin, Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F19%3A63523649" target="_blank" >RIV/70883521:28110/19:63523649 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.earthdoc.org/content/papers/10.3997/2214-4609.201902885" target="_blank" >https://www.earthdoc.org/content/papers/10.3997/2214-4609.201902885</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isotopic and microbiological evidence of microbial methane and CO2 in the Upper Silesian Coal Basin, Czech Republic

Popis výsledku v původním jazyce

Czech part of the Upper Silesian Coal Basin (USCB) is an outstanding natural laboratory with examples of both thermogenic and microbial methane associated with bituminous coal seams. Current research is aimed at providing geochemical and biochemical evidence of past and present activity of methanogenic and methanotrophic archea in the USCB basin. The thermogenic methane occurs in the USCB in the areas covered by the impermeable sediments of the Carpathian Flysch Belt protecting Carboniferous strata from descendent pore waters and act as a barrier for the migration of methane gas to the surface. In addition to previous data (e.g. by Weniger et al. 2012), new measurements of coal-seam gases in the Namurian and Westphalian contain 40–96 % of methane with the d13C value ranging from -33 to -53 ‰. Microbial methane occurs in Namurian A and B strata in the northern segments of the Czech and mainly Polish part of the USCB (Kotarba 2001), outside the Carpathian fold-and-thrust belt. Its d13C values range from -55 to -88 ‰. Ample sampling revealed details in coal-bed gas composition with respect to fault tectonics and geological position. CO2 δ13C ranges from -5 to -27 ‰, but in some places from -30 to - 50 ‰, pointing to microbial oxidation of methane. Application of PCR provided deeper insight into methanogen and methanotrophic consortia dynamics in coal-bed methane related environment. PCR (polymerase chain reaction) was used for sample screening followed by qPCR (quantitative polymerase chain reaction) determination of abundances of bacteria, archaea and methanogenes. Positive signal of mcrA gene presence was confirmed, though clear relationship to methanotrophs needs more investigation. Isotopic, chemical and microbiological data suggest presence of active microbial methanogenesis in the NE part of the Upper Silesian Coal Basin in the Czech Republic.

Název v anglickém jazyce

Isotopic and microbiological evidence of microbial methane and CO2 in the Upper Silesian Coal Basin, Czech Republic

Popis výsledku anglicky

Czech part of the Upper Silesian Coal Basin (USCB) is an outstanding natural laboratory with examples of both thermogenic and microbial methane associated with bituminous coal seams. Current research is aimed at providing geochemical and biochemical evidence of past and present activity of methanogenic and methanotrophic archea in the USCB basin. The thermogenic methane occurs in the USCB in the areas covered by the impermeable sediments of the Carpathian Flysch Belt protecting Carboniferous strata from descendent pore waters and act as a barrier for the migration of methane gas to the surface. In addition to previous data (e.g. by Weniger et al. 2012), new measurements of coal-seam gases in the Namurian and Westphalian contain 40–96 % of methane with the d13C value ranging from -33 to -53 ‰. Microbial methane occurs in Namurian A and B strata in the northern segments of the Czech and mainly Polish part of the USCB (Kotarba 2001), outside the Carpathian fold-and-thrust belt. Its d13C values range from -55 to -88 ‰. Ample sampling revealed details in coal-bed gas composition with respect to fault tectonics and geological position. CO2 δ13C ranges from -5 to -27 ‰, but in some places from -30 to - 50 ‰, pointing to microbial oxidation of methane. Application of PCR provided deeper insight into methanogen and methanotrophic consortia dynamics in coal-bed methane related environment. PCR (polymerase chain reaction) was used for sample screening followed by qPCR (quantitative polymerase chain reaction) determination of abundances of bacteria, archaea and methanogenes. Positive signal of mcrA gene presence was confirmed, though clear relationship to methanotrophs needs more investigation. Isotopic, chemical and microbiological data suggest presence of active microbial methanogenesis in the NE part of the Upper Silesian Coal Basin in the Czech Republic.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

29th International Meeting on Organic Geochemistry, IMOG 2019

ISBN

978-94-6282-304-4

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

2

Název nakladatele

European Association of Geoscientists and Engineers, EAGE

Místo vydání

Houten

Místo konání akce

Gothenburg

Datum konání akce

1. 9. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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