Production of Polyhydroxyalkanoates in Bacterial Isolates from Food

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63570118" target="_blank" >RIV/70883521:28110/23:63570118 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Production of Polyhydroxyalkanoates in Bacterial Isolates from Food

Popis výsledku v původním jazyce

Polyhydroxyalkanoates (PHAs) have extensive applications in the medical field and potentially in other sectors due to their biocompatibility and biodegradability. Gaining insights into the biosynthetic pathways of PHAs could facilitate the identification of optimal conditions (substrates) for producing specific types of PHAs using particular microbial strains. This research aimed to develop a methodology for identifying potentially valuable PHA-producing bacteria. Within this study, PCR analysis revealed the presence of all four classes of PHA synthases and other genes involved in PHA formation (fabG, phaA, phaB, phaG, and phaJ) in 64 bacterial collection strains and food isolates. The strains encompassed various genera, including Acinetobacter, Bacillus, Cupriavidus, Escherichia, Klebsiella, Lelliottia, Lysinibacillus, Mammaliicoccus, Oceanobacillus, Pantoea, Peribacillus, Priestia, Pseudomonas, Rahnella, Staphylococcus, and Stenotrophomonas. Carbon sources such as fructose, glucose, sunflower oil, and propionic acid were utilized, and PHA production was assessed through Sudan black staining, Nile blue staining, and FTIR techniques. Class I synthase and phaA genes were most frequently observed, indicating the strains&apos; capacity for synthesizing PHA from carbohydrates. Among the bacterial strains tested, the Pseudomonas genus demonstrated the ability to utilize all carbon sources examined. Notably, the Pseudomonas extremorientalis strain exhibited potential for biotechnological applications.

Název v anglickém jazyce

Production of Polyhydroxyalkanoates in Bacterial Isolates from Food

Popis výsledku anglicky

Polyhydroxyalkanoates (PHAs) have extensive applications in the medical field and potentially in other sectors due to their biocompatibility and biodegradability. Gaining insights into the biosynthetic pathways of PHAs could facilitate the identification of optimal conditions (substrates) for producing specific types of PHAs using particular microbial strains. This research aimed to develop a methodology for identifying potentially valuable PHA-producing bacteria. Within this study, PCR analysis revealed the presence of all four classes of PHA synthases and other genes involved in PHA formation (fabG, phaA, phaB, phaG, and phaJ) in 64 bacterial collection strains and food isolates. The strains encompassed various genera, including Acinetobacter, Bacillus, Cupriavidus, Escherichia, Klebsiella, Lelliottia, Lysinibacillus, Mammaliicoccus, Oceanobacillus, Pantoea, Peribacillus, Priestia, Pseudomonas, Rahnella, Staphylococcus, and Stenotrophomonas. Carbon sources such as fructose, glucose, sunflower oil, and propionic acid were utilized, and PHA production was assessed through Sudan black staining, Nile blue staining, and FTIR techniques. Class I synthase and phaA genes were most frequently observed, indicating the strains&apos; capacity for synthesizing PHA from carbohydrates. Among the bacterial strains tested, the Pseudomonas genus demonstrated the ability to utilize all carbon sources examined. Notably, the Pseudomonas extremorientalis strain exhibited potential for biotechnological applications.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů