Self-crosslinkable bacterial cellulose/chitosan/pectin injectable hydrogels: Design, characterization and preliminary biological performance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F25%3A63597139" target="_blank" >RIV/70883521:28110/25:63597139 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/25:63597139

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/app.57752" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/app.57752</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/app.57752" target="_blank" >10.1002/app.57752</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Self-crosslinkable bacterial cellulose/chitosan/pectin injectable hydrogels: Design, characterization and preliminary biological performance

Popis výsledku v původním jazyce

In this study, multifunctional polysaccharide-based injectable hydrogels were developed using chitosan (CS) and dialdehyde bacterial cellulose (D-BC), interpenetrated with pectin (PT). The hydrogels exhibited rapid gelation, good water retention, and injectability under physiological conditions. Comprehensive characterization was performed to assess their chemical structure, internal morphology, thermal stability, and rheological behavior. The formation of dynamic Schiff base bonds between amine groups of CS and aldehyde groups of D-BC facilitated efficient crosslinking, resulting in rapid gelation and favorable swelling properties. The hydrogels also demonstrated shear-thinning behavior, contributing to their injectable and self-supporting characteristics. In vitro biocompatibility was evaluated over 21 days using gingival mesenchymal stem cells (GMSCs), with all formulations maintaining over 80% cell viability, confirming their cytocompatibility. Antibacterial assays revealed significant inhibition of Staphylococcus aureus, indicating promising antimicrobial performance. The 3D hydrogel networks provided a porous and stable structure suitable for cellular infiltration and tissue integration. Overall, this work presents a green, bio-based approach for fabricating injectable hydrogels with tunable physicochemical and biological properties, offering a potential platform for soft tissue repair applications, particularly in maxillofacial regeneration.

Název v anglickém jazyce

Self-crosslinkable bacterial cellulose/chitosan/pectin injectable hydrogels: Design, characterization and preliminary biological performance

Popis výsledku anglicky

In this study, multifunctional polysaccharide-based injectable hydrogels were developed using chitosan (CS) and dialdehyde bacterial cellulose (D-BC), interpenetrated with pectin (PT). The hydrogels exhibited rapid gelation, good water retention, and injectability under physiological conditions. Comprehensive characterization was performed to assess their chemical structure, internal morphology, thermal stability, and rheological behavior. The formation of dynamic Schiff base bonds between amine groups of CS and aldehyde groups of D-BC facilitated efficient crosslinking, resulting in rapid gelation and favorable swelling properties. The hydrogels also demonstrated shear-thinning behavior, contributing to their injectable and self-supporting characteristics. In vitro biocompatibility was evaluated over 21 days using gingival mesenchymal stem cells (GMSCs), with all formulations maintaining over 80% cell viability, confirming their cytocompatibility. Antibacterial assays revealed significant inhibition of Staphylococcus aureus, indicating promising antimicrobial performance. The 3D hydrogel networks provided a porous and stable structure suitable for cellular infiltration and tissue integration. Overall, this work presents a green, bio-based approach for fabricating injectable hydrogels with tunable physicochemical and biological properties, offering a potential platform for soft tissue repair applications, particularly in maxillofacial regeneration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10620 - Other biological topics

Projekt

<a href="/cs/project/TH71020005" target="_blank" >TH71020005: Bioaktivní vstřikovatelné hydrogely pro regeneraci měkkých tkání po rekonstrukčních maxilofaciálních operacích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Polymer Science

ISSN

0021-8995

e-ISSN

1097-4628

Svazek periodika

142

Číslo periodika v rámci svazku

45

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

nestránkováno

Kód UT WoS článku

001540648600001

EID výsledku v databázi Scopus

2-s2.0-105012153501