The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F25%3A63597519" target="_blank" >RIV/70883521:28110/25:63597519 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00144641 RIV/60461373:22810/25:43933006

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141813025085162?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813025085162?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2025.147959" target="_blank" >10.1016/j.ijbiomac.2025.147959</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films

Popis výsledku v původním jazyce

The development of composites with tailored surface properties and electrical conductivity is critical for various biomedical applications. However, a substantial gap remains in understanding how the unique properties of cellulose, such as biocompatibility and renewability can be effectively combined with those of polyaniline (PANI), including electrical conductivity, antibacterial, and antioxidant activity, to create composite films with advanced multifunctional performance. Indeed, conductivity can be used not only to monitor biological functions but also as a cell instructive factor. To meet these requirements, thin composite films were synthetized using oxidative polymerization of aniline hydrochloride with ammonium peroxydisulfate in the presence of either cellulose nanocrystals (CNC) or cellulose nanofibres (CNF). Their cytocompatibility was demonstrated with the NIH/3 T3 fibroblast line and highly progressive human induced pluripotent stem cells. The films also showed antibacterial activity against Staphyloccocus aureus and Escherichia coli, surpassing that of pristine PANI and meeting the EN ISO 20743 criteria for materials with significant activity (reducing CFU value to zero). Comprehensive physicochemical characterization revealed that the films possessed exceptional DPPH radical scavenging achieving their complete (100 %) removal within 15 min, and electrical conductivity within units of S cm−1. Raman spectroscopy showed that PANI/CNC composites were more resistant to deprotonation caused by laser illumination than PANI/CNF, which resulted from the presence of sulfate groups on the CNC surface. These findings highlight that PANI/CNC and PANI/CNF films are promising materials for applications requiring surfaces that are simultaneously biocompatible, electrically conductive, and antibacterial.

Název v anglickém jazyce

The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films

Popis výsledku anglicky

The development of composites with tailored surface properties and electrical conductivity is critical for various biomedical applications. However, a substantial gap remains in understanding how the unique properties of cellulose, such as biocompatibility and renewability can be effectively combined with those of polyaniline (PANI), including electrical conductivity, antibacterial, and antioxidant activity, to create composite films with advanced multifunctional performance. Indeed, conductivity can be used not only to monitor biological functions but also as a cell instructive factor. To meet these requirements, thin composite films were synthetized using oxidative polymerization of aniline hydrochloride with ammonium peroxydisulfate in the presence of either cellulose nanocrystals (CNC) or cellulose nanofibres (CNF). Their cytocompatibility was demonstrated with the NIH/3 T3 fibroblast line and highly progressive human induced pluripotent stem cells. The films also showed antibacterial activity against Staphyloccocus aureus and Escherichia coli, surpassing that of pristine PANI and meeting the EN ISO 20743 criteria for materials with significant activity (reducing CFU value to zero). Comprehensive physicochemical characterization revealed that the films possessed exceptional DPPH radical scavenging achieving their complete (100 %) removal within 15 min, and electrical conductivity within units of S cm−1. Raman spectroscopy showed that PANI/CNC composites were more resistant to deprotonation caused by laser illumination than PANI/CNF, which resulted from the presence of sulfate groups on the CNC surface. These findings highlight that PANI/CNC and PANI/CNF films are promising materials for applications requiring surfaces that are simultaneously biocompatible, electrically conductive, and antibacterial.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

<a href="/cs/project/GA23-07425S" target="_blank" >GA23-07425S: Anisotropní a elektricky vodivé biomateriály</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

330

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

nestránkováno

Kód UT WoS článku

001592624800001

EID výsledku v databázi Scopus

2-s2.0-105017731319