Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28140%2F18%3A63520516" target="_blank" >RIV/70883521:28140/18:63520516 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/18:PU130843 RIV/68407700:21230/19:00325904

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0925400518321919" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400518321919</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2018.12.072" target="_blank" >10.1016/j.snb.2018.12.072</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Popis výsledku v původním jazyce

We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.

Název v anglickém jazyce

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Popis výsledku anglicky

We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

<a href="/cs/project/GA16-11140S" target="_blank" >GA16-11140S: Na mikrofludice založená velmi rychlá diferenční fluometrie pro výzkum léků (µDSF)</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SENSORS AND ACTUATORS B-CHEMICAL

ISSN

0925-4005

e-ISSN

—

Svazek periodika

2018

Číslo periodika v rámci svazku

283

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

677-684

Kód UT WoS článku

000455854000082

EID výsledku v databázi Scopus

2-s2.0-85058703025