Selected Spectrometric Metrods for Determination of Iodine in Biological Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28160%2F22%3A63557380" target="_blank" >RIV/70883521:28160/22:63557380 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/22:63557380

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Selected Spectrometric Metrods for Determination of Iodine in Biological Samples

Popis výsledku v původním jazyce

This article compares different methods for determination of iodine in biological samples. Most frequently, indirect methods are applied in clinical practice. Sandell-Kolthoff spectrophotometric method of iodine determination [1] ranks among the most widely used ones; researchers also developed other modifications of the above method. Regarding direct determination, the following methods are considered the most suitable ones: emission spectrometry with inductively coupled plasma (ICP-OES), electrochemical methods, mass spectrometry with inductively coupled plasma (ICP-MS), and nuclear activation analysis (NAA) [2]. The ICP-MS and NAA are costly, but iodine can be analyzed directly regardless of its form, oxidation state, and isotopic distribution of iodine [3]. On the contrary, electrochemical, and ICP-OES determinations can be complicated with remarkable spectral interferences and also with dependence on possible presence of various forms and oxidation states of iodine in samples [4].We compared three most frequently used methods for determination of iodine in selected biological materials: Sandell-Kolthoff spectrophotometric determination, ICP-OES and ICP-MS techniques [5]. The samples of biological materials such as foods and food supplements corresponding to content of iodine in urine were selected (Jooste and Strydom 2010). A NIST-SRM-1549 Milk powder certified reference material (CRM) with certified iodine concentration (3.38+0.6 mg/kg) was used to control the accuracy of the total iodine determination. The least accurate results were obtained by ICP-OES in analyses of given CRM (5.64+0.39 mg/kg), better results were acquired by spectrophotometric method (4.53+0.23 mg/kg) and the most accurate results were provided by ICP-MS method (3.36+0.12 mg/kg of iodine).

Název v anglickém jazyce

Selected Spectrometric Metrods for Determination of Iodine in Biological Samples

Popis výsledku anglicky

This article compares different methods for determination of iodine in biological samples. Most frequently, indirect methods are applied in clinical practice. Sandell-Kolthoff spectrophotometric method of iodine determination [1] ranks among the most widely used ones; researchers also developed other modifications of the above method. Regarding direct determination, the following methods are considered the most suitable ones: emission spectrometry with inductively coupled plasma (ICP-OES), electrochemical methods, mass spectrometry with inductively coupled plasma (ICP-MS), and nuclear activation analysis (NAA) [2]. The ICP-MS and NAA are costly, but iodine can be analyzed directly regardless of its form, oxidation state, and isotopic distribution of iodine [3]. On the contrary, electrochemical, and ICP-OES determinations can be complicated with remarkable spectral interferences and also with dependence on possible presence of various forms and oxidation states of iodine in samples [4].We compared three most frequently used methods for determination of iodine in selected biological materials: Sandell-Kolthoff spectrophotometric determination, ICP-OES and ICP-MS techniques [5]. The samples of biological materials such as foods and food supplements corresponding to content of iodine in urine were selected (Jooste and Strydom 2010). A NIST-SRM-1549 Milk powder certified reference material (CRM) with certified iodine concentration (3.38+0.6 mg/kg) was used to control the accuracy of the total iodine determination. The least accurate results were obtained by ICP-OES in analyses of given CRM (5.64+0.39 mg/kg), better results were acquired by spectrophotometric method (4.53+0.23 mg/kg) and the most accurate results were provided by ICP-MS method (3.36+0.12 mg/kg of iodine).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/ED2.1.00%2F03.0111" target="_blank" >ED2.1.00/03.0111: Centrum polymerních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů