Improvement of Temozolomide stability by loading in Chitosan-carboxy enriched Polylactide nanoparticles. In vitro evaluation.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F16%3A43875561" target="_blank" >RIV/70883521:28610/16:43875561 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of Temozolomide stability by loading in Chitosan-carboxy enriched Polylactide nanoparticles. In vitro evaluation.

Popis výsledku v původním jazyce

Temozolomide (TMZ) is a chemotherapeutic agent, which has demonstrated promising activity in treatment of some brain cancers and other solid tumors [1]. However, treatment is still a challenge due to the fast degradation of TMZ in the active form MTIC (5-Monomethyltriazenoimidazole-4-carboxamide) which is not able to reach the tumor site, in therapeutic concentration [2]. In this work, TMZ was physically entrapped into an amphiphilic carrier prepared by grafting carboxy- enriched polylactide to chitosan backbone. Obtained nanoparticles demonstrate diameter in the range 100-150 nm, high stability in physiological media and ability to accommodate up to 800 g of TMZ per mg of polymer. TMZ loaded in CS-PLA showed higher stability over time in comparison with the free drug, in particular in physiological solution at pH 7.4. In vitro cell tests demonstrate improvement of TMZ effectiveness when loaded into the carrier compared to the free drug counterparts.

Název v anglickém jazyce

Improvement of Temozolomide stability by loading in Chitosan-carboxy enriched Polylactide nanoparticles. In vitro evaluation.

Popis výsledku anglicky

Temozolomide (TMZ) is a chemotherapeutic agent, which has demonstrated promising activity in treatment of some brain cancers and other solid tumors [1]. However, treatment is still a challenge due to the fast degradation of TMZ in the active form MTIC (5-Monomethyltriazenoimidazole-4-carboxamide) which is not able to reach the tumor site, in therapeutic concentration [2]. In this work, TMZ was physically entrapped into an amphiphilic carrier prepared by grafting carboxy- enriched polylactide to chitosan backbone. Obtained nanoparticles demonstrate diameter in the range 100-150 nm, high stability in physiological media and ability to accommodate up to 800 g of TMZ per mg of polymer. TMZ loaded in CS-PLA showed higher stability over time in comparison with the free drug, in particular in physiological solution at pH 7.4. In vitro cell tests demonstrate improvement of TMZ effectiveness when loaded into the carrier compared to the free drug counterparts.

Druh

O - Ostatní výsledky

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů