The Effect of High Temperatures on Antibacterial Activity and Structural Changes of Pla-Based Nisin-Containing Films

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F18%3A63521340" target="_blank" >RIV/70883521:28610/18:63521340 - isvavai.cz</a>

Výsledek na webu

<a href="http://cps.utb.cz/images/aktuality/Sborn%C3%ADk_PLASTKO_2018.pdf" target="_blank" >http://cps.utb.cz/images/aktuality/Sborn%C3%ADk_PLASTKO_2018.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of High Temperatures on Antibacterial Activity and Structural Changes of Pla-Based Nisin-Containing Films

Popis výsledku v původním jazyce

The use of bacteriocins as natural compounds to generate active plastic materials has received considerable attention in the last decade. The most well-known bacteriocin, nisin has been used for the modification of many different polymers. However, only few studies have investigated formulating nisin-incorporated biodegradable films by means of thermoplastic processing relating to the sensitivity of nisin as a protein to high temperatures and the loss of its efficacy against pathogenic microorganisms. The presented work investigates the thermal stability of bacteriocin nisin in polylactide (PLA) and polylactide/polyethylene glycol (PLA/PEG) films at temperatures approaching the processing temperature of PLA. The samples were prepared by solvent cast technique and characterized according to their thermal, structural and antibacterial properties. Research on the thermal stability of nisin in the PLA and PLA/PEG systems was carried out by exposing the given films to high temperatures (160°C and 180°C) for a duration of up to 4 h. Assessment of the antibacterial activity of the samples was carried out by the agar diffusion method against Micrococcus luteus, while the structural changes in the polymer matrix were evaluated by gel permeation chromatography. The results showed that nisin retained almost 70% of its antimicrobial activity in the PLA matrix, even after treatment at 160°C for 15 min. The presence of PEG significantly increased the degradation of nisin as well as it enhanced the degradation of the PLA matrix.

Název v anglickém jazyce

The Effect of High Temperatures on Antibacterial Activity and Structural Changes of Pla-Based Nisin-Containing Films

Popis výsledku anglicky

The use of bacteriocins as natural compounds to generate active plastic materials has received considerable attention in the last decade. The most well-known bacteriocin, nisin has been used for the modification of many different polymers. However, only few studies have investigated formulating nisin-incorporated biodegradable films by means of thermoplastic processing relating to the sensitivity of nisin as a protein to high temperatures and the loss of its efficacy against pathogenic microorganisms. The presented work investigates the thermal stability of bacteriocin nisin in polylactide (PLA) and polylactide/polyethylene glycol (PLA/PEG) films at temperatures approaching the processing temperature of PLA. The samples were prepared by solvent cast technique and characterized according to their thermal, structural and antibacterial properties. Research on the thermal stability of nisin in the PLA and PLA/PEG systems was carried out by exposing the given films to high temperatures (160°C and 180°C) for a duration of up to 4 h. Assessment of the antibacterial activity of the samples was carried out by the agar diffusion method against Micrococcus luteus, while the structural changes in the polymer matrix were evaluated by gel permeation chromatography. The results showed that nisin retained almost 70% of its antimicrobial activity in the PLA matrix, even after treatment at 160°C for 15 min. The presence of PEG significantly increased the degradation of nisin as well as it enhanced the degradation of the PLA matrix.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů